Paradoxical binding levels of vasoactive amines to cultured cerebral microvessel derived endothelial cells
Vascular sensitization to vasoactive amines (VAA) may be critical for the development of experimental autoimmune encephalitis as well as other autoimmune diseases. Some inbred stains of mice such as SJL/J are particularly sensitive to the effects of VAA while others (BALB/c) are not. This study was performed to determine if the differing response to VAA in vivo is due to differing levels of binding of VAA to cultured brain endothelial (En) cells in vitro. Cells were isolated, grown to confluence, washed twice with binding buffer and incubated with either /sup 3/H-histamine, /sup 3/H-mepyramine or /sup 3/H-5 hydroxytryptamine (5HT) for 1 hour at 37/sup 0/C. Results showed that the BALB derived En cells specifically bound approximately twice as much mepyramine and three times as much 5-HT as the SJL derived En cells. The relative low binding of VAA to SJL En cells may reflect the extreme in vivo sensitivity that this mouse strain displays toward VAA. These seemingly paradoxical levels of VAA binding in the cultured cerebral endothelium may be due to genetic factors and may give insight into diseases that affect the blood brain barrier.
- Research Organization:
- Univ. of Iowa, Iowa City
- OSTI ID:
- 5383278
- Report Number(s):
- CONF-8604222-; TRN: 86-026735
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
A potent and selective C-11 labeled PET tracer for imaging sphingosine-1-phosphate receptor 2 in the CNS demonstrates sexually dimorphic expression
Buspirone and gepirone: partial agonists at the 5HT/sub 1/A receptor linked to adenylate cyclase (AC) in rat and guinea pig hippocampal preparations
Related Subjects
AMINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL VARIABILITY
BLOOD-BRAIN BARRIER
BRAIN
CELL CULTURES
ENCEPHALITIS
HISTAMINE
MICE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TRYPTAMINES
ANIMALS
AROMATICS
AZAARENES
AZOLES
BODY
CENTRAL NERVOUS SYSTEM
DISEASES
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
INDOLES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
NERVOUS SYSTEM
NERVOUS SYSTEM DISEASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRROLES
REACTION KINETICS
RODENTS
VERTEBRATES
VIRAL DISEASES
550901* - Pathology- Tracer Techniques