Altered lauric acid metabolism in renal microsomes from spontaneously hypertensive rats (SHR)
Studies investigated whether changes in omega- and (omega-1)-hydroxylation (OH) of lauric acid (LA) occurred in renal microsomes prepared from SHR compared to Wistar-Kyoto (WK) control rats. Systolic blood pressure in age-matched adult SHR and WKR were 189 +/- 3 and 123 +/- 4 mm Hg(anti X +/- SE) respectively (p < 0.001). No significant differences between SHR and WKR were seen in body weight, kidney weight or renal microsomal protein content. Renal microsomes, prepared from whole kidneys, were incubated with 10 mM NADPH and (/sup 14/C)LA at concentrations between 5-50 ..mu..M. The 11- and 12-OH metabolites of LA were separated by HPLC using a reverse phase column with a methanol:water:acetic acid (62:37.8:0.2) mobile phase. Apparent (app) V/sub max/ values for 12-OH in WKR and SHR were 0.87 +/- 0.19 vs 1.48 +/- .11 nmoles/mg protein/min (p < 0.05), respectively, while values for 11-OH were 0.51 +/- 0.12 vs 0.60 +/- .07, respectively. No significant differences were found in app K/sub m/ values for either 11- or 12-OH between the two strains. SDS-polyacrylamide gel electrophoresis of renal microsomes showed the increased prominence of a 52,000 dalton protein in SHR preparations. Thus data suggest that selective alterations in renal cytochrome P-450 monooxygenase reactions may be associated with the endogenous biochemical processes underlying hypertension.
- Research Organization:
- Univ. of Florida, Gainesville
- OSTI ID:
- 5383269
- Report Number(s):
- CONF-8604222-; TRN: 86-026736
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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METABOLISM
HYPERTENSION
PATHOGENESIS
BLOOD PRESSURE
CARBON 14 COMPOUNDS
ELECTROPHORESIS
KIDNEYS
LIQUID COLUMN CHROMATOGRAPHY
METABOLITES
MICROSOMES
NADP
OXYGENASES
RATS
TRACER TECHNIQUES
WEIGHT
ANIMALS
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CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CELL CONSTITUENTS
CHROMATOGRAPHY
COENZYMES
DISEASES
ENZYMES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
RODENTS
SEPARATION PROCESSES
SYMPTOMS
VASCULAR DISEASES
VERTEBRATES
550901* - Pathology- Tracer Techniques