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Title: Mortality of fecal bacteria in seawater

Abstract

The authors propose a method for determining the mortality rate for allochthonous bacteria released in aquatic environments without interference due to the loss of culturability in specific culture media. This method consists of following the disappearance of radioactivity from the trichloracetic acid-insoluble fraction in water samples to which ({sup 3}H)thymidine-prelabeled allochthonous bacteria have been added. In coastal seawater, they found that the actual rate of disappearance of fecal bacteria was 1 order of magnitude lower than the rate of loss of culturability on specific media. Minor adaptation of the procedure may facilitate assessment of the effect of protozoan grazing and bacteriophage lysis on the overall bacterial mortality rate.

Authors:
; ; ;  [1]
  1. Univ. Libre de Bruxelles, Brussels (Belgium)
Publication Date:
OSTI Identifier:
5382463
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology; (United States)
Additional Journal Information:
Journal Volume: 57:3; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; PATHOGENS; MORTALITY; SEAWATER; WATER QUALITY; BACTERIOPHAGES; CULTURE MEDIA; PROTOZOA; THYMIDINE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMALS; AZINES; ENVIRONMENTAL QUALITY; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; INVERTEBRATES; ISOTOPE APPLICATIONS; MICROORGANISMS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; OXYGEN COMPOUNDS; PARASITES; PYRIMIDINES; RIBOSIDES; VIRUSES; WATER; 540311* - Environment, Aquatic- Basic Studies- Radiometric Techniques- (1990-)

Citation Formats

Garcia-Lara, J, Menon, P, Servais, P, and Billen, G. Mortality of fecal bacteria in seawater. United States: N. p., 1991. Web.
Garcia-Lara, J, Menon, P, Servais, P, & Billen, G. Mortality of fecal bacteria in seawater. United States.
Garcia-Lara, J, Menon, P, Servais, P, and Billen, G. 1991. "Mortality of fecal bacteria in seawater". United States.
@article{osti_5382463,
title = {Mortality of fecal bacteria in seawater},
author = {Garcia-Lara, J and Menon, P and Servais, P and Billen, G},
abstractNote = {The authors propose a method for determining the mortality rate for allochthonous bacteria released in aquatic environments without interference due to the loss of culturability in specific culture media. This method consists of following the disappearance of radioactivity from the trichloracetic acid-insoluble fraction in water samples to which ({sup 3}H)thymidine-prelabeled allochthonous bacteria have been added. In coastal seawater, they found that the actual rate of disappearance of fecal bacteria was 1 order of magnitude lower than the rate of loss of culturability on specific media. Minor adaptation of the procedure may facilitate assessment of the effect of protozoan grazing and bacteriophage lysis on the overall bacterial mortality rate.},
doi = {},
url = {https://www.osti.gov/biblio/5382463}, journal = {Applied and Environmental Microbiology; (United States)},
issn = {0099-2240},
number = ,
volume = 57:3,
place = {United States},
year = {Fri Mar 01 00:00:00 EST 1991},
month = {Fri Mar 01 00:00:00 EST 1991}
}