Release of histamine from mast cells by a synthetic ionophore
Polyphore 27:10 is a copolymer composed of blocks of polyoxyethylene and polyoxypropylene. Subcutaneous injection of this copolymer in mice causes significant inflammation as determined by footpad swelling and increased vascular permeability as measured by extravasation of Evan's blue dye. Micromolar concentrations trigger release of histamine from purified mouse mast cells and human peripheral blood basophils in vitro. Histamine release from murine mast cells required physiologic temperature, metabolic energy, and calcium ions in the surrounding medium. Replacement of sodium ions in the medium with either potassium or lithium markedly inhibited release. Since Polyphore 27:10 has structural homology with ethylenediamine tetraacetic acid (EDTA) and the crown polyethers, both of which chelate metal ions, The authors investigated whether Polyphore 27:10 could act as an ionophore. The Polyphore transported cations across artificial lipid membranes and made erythrocytes highly permeable to sodium ions, but not calcium at physiologic concentrations. They propose that the potent inflammatory and histamine-releasing activities of this copolymer are in part due to its ability to depolarize and degranulate mast cells.
- Research Organization:
- Emory Univ., Atlanta, GA
- OSTI ID:
- 5381949
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL MEMBRANES
PERMEABILITY
HISTAMINE
SECRETION
MAST CELLS
INFLAMMATION
ORGANIC POLYMERS
BIOLOGICAL EFFECTS
BASOPHILS
MAN
MICE
AMINES
ANIMAL CELLS
ANIMALS
AZOLES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGICAL CHANGES
POLYMERS
PRIMATES
RODENTS
SOMATIC CELLS
SYMPTOMS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)