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Title: Enhanced unscheduled DNA synthesis in UV-irradiated human skin explants treated with T4N5 liposomes

Abstract

Epidermal keratinocytes cultured from explants of skin cancer patients, including biopsies from xeroderma pigmentosum patients, were ultraviolet light-irradiated and DNA repair synthesis was measured. Repair capacity was much lower in xeroderma pigmentosum patients than in normal patients. The extent of DNA repair replication did not decline with the age of the normal patient. Treatment with T4N5 liposomes containing a DNA repair enzyme enhanced repair synthesis in both normal and xeroderma pigmentosum keratinocytes in an irradiation- and liposome-dose dependent manner. These results provide no evidence that aging people or skin cancer patients are predisposed to cutaneous malignancy by a DNA repair deficiency, but do demonstrate that T4N5 liposomes enhance DNA repair in the keratinocytes of the susceptible xeroderma pigmentosum and skin cancer population.

Authors:
; ; ;  [1]
  1. Applied Genetics Inc., Freeport, NY (USA)
Publication Date:
OSTI Identifier:
5380747
Resource Type:
Journal Article
Journal Name:
Journal of Investigative Dermatology; (United States)
Additional Journal Information:
Journal Volume: 97:1; Journal ID: ISSN 0022-202X
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANIMAL CELLS; DNA REPAIR; LIPOSOMES; RADIOSENSITIVITY EFFECTS; SKIN; TRANSPLANTS; BIOLOGICAL RADIATION EFFECTS; MAN; NEOPLASMS; PATIENTS; ULTRAVIOLET RADIATION; XERODERMA PIGMENTOSUM; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; BODY; DISEASES; ELECTROMAGNETIC RADIATION; MAMMALS; ORGANS; PRIMATES; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; SKIN DISEASES; VERTEBRATES; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture

Citation Formats

Yarosh, D B, Kibitel, J T, Green, L A, and Spinowitz, A. Enhanced unscheduled DNA synthesis in UV-irradiated human skin explants treated with T4N5 liposomes. United States: N. p., 1991. Web. doi:10.1111/1523-1747.ep12479314.
Yarosh, D B, Kibitel, J T, Green, L A, & Spinowitz, A. Enhanced unscheduled DNA synthesis in UV-irradiated human skin explants treated with T4N5 liposomes. United States. https://doi.org/10.1111/1523-1747.ep12479314
Yarosh, D B, Kibitel, J T, Green, L A, and Spinowitz, A. 1991. "Enhanced unscheduled DNA synthesis in UV-irradiated human skin explants treated with T4N5 liposomes". United States. https://doi.org/10.1111/1523-1747.ep12479314.
@article{osti_5380747,
title = {Enhanced unscheduled DNA synthesis in UV-irradiated human skin explants treated with T4N5 liposomes},
author = {Yarosh, D B and Kibitel, J T and Green, L A and Spinowitz, A},
abstractNote = {Epidermal keratinocytes cultured from explants of skin cancer patients, including biopsies from xeroderma pigmentosum patients, were ultraviolet light-irradiated and DNA repair synthesis was measured. Repair capacity was much lower in xeroderma pigmentosum patients than in normal patients. The extent of DNA repair replication did not decline with the age of the normal patient. Treatment with T4N5 liposomes containing a DNA repair enzyme enhanced repair synthesis in both normal and xeroderma pigmentosum keratinocytes in an irradiation- and liposome-dose dependent manner. These results provide no evidence that aging people or skin cancer patients are predisposed to cutaneous malignancy by a DNA repair deficiency, but do demonstrate that T4N5 liposomes enhance DNA repair in the keratinocytes of the susceptible xeroderma pigmentosum and skin cancer population.},
doi = {10.1111/1523-1747.ep12479314},
url = {https://www.osti.gov/biblio/5380747}, journal = {Journal of Investigative Dermatology; (United States)},
issn = {0022-202X},
number = ,
volume = 97:1,
place = {United States},
year = {Mon Jul 01 00:00:00 EDT 1991},
month = {Mon Jul 01 00:00:00 EDT 1991}
}