Effects of combined butylated hydroxyanisole and 3-methylcholanthrene treatments on the expression of p-nitro-phenol UDP-glucuronyltransferase
UDP-glucuronyltransferase (UDP-GT) is a very important microsomal xenobiotic detoxicating enzyme. This enzyme has been shown to be induced by a variety of xenobiotics including the carcinogen, 3-methylcholanthrene (3-MC) and the phenolic antioxidant (BHA) which is a widely used food additive. Recently, our laboratory demonstrated that simultaneous administration of 3-MC and BHA to rats results to a synergistic induction of the biotransformation enzymes including UDP-GT. We have determined the elution profile of this enzyme and its multiple forms on ion-exchange and affinity chromatography columns. To further understand the expression of this particular enzyme, we have isolated poly (A/sup +/) RNA from BHA + 3-MC induced rat livers, and in vitro translated them to demonstrate the presence of a protein similar to the authentic UDP-GT in activity and molecular weight, as well as generated cDNA library from them. We hope to immunoprecipitate specific p-nitrophenol UDP-GT from the in vitro translated poly (A/sup +/) RNA and isolate specific mRNA by polysome immunoadsorption in order to generate cDNA to further characterize this unique and important enzyme.
- Research Organization:
- Atlanta Univ., GA (USA)
- OSTI ID:
- 5378065
- Journal Information:
- Ga. J. Sci.; (United States), Vol. 45:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
3-METHYLCHOLANTHRENE
BIOLOGICAL EFFECTS
ANISOLE
NITROPHENOL
TRANSFERASES
ENZYME INDUCTION
HYDROXY COMPOUNDS
LIVER
RATS
RNA
SYNERGISM
ANIMALS
AROMATICS
BODY
DIGESTIVE SYSTEM
ENZYMES
ETHERS
GENE REGULATION
GLANDS
HYDROCARBONS
MAMMALS
NITRO COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PHENOLS
POLYCYCLIC AROMATIC HYDROCARBONS
RODENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology