Cellular response to exogenous electromagnetic fields. Annual report, October 1986-September 1987
This research has determined that extremely-low-frequency (ELF) (100-Hz) electromagnetic (EM) fields induce alterations in transcription and translation in dipteran (Drosophila, Sciara) and human (HL60, IB4) cells. Transcriptional autoradiography and endoreplicated chromosomes, as well as analyses of sucrose density-gradient profiles, shows increased uptake of /sup 3/H uridine into RNA following exposure of cells to sinusoidal and asymmetric quasi-rectangular signals for time periods up to 60 minutes. Biochemical analyses (sucrose density gradients, oligo (d) T columns) have demonstrated that the RNA affected by signal stimulation is in the messenger-RNA size class. Quantitative and qualitative alterations also occur in polypeptide synthesis following EM-field stimulation. Major differences are noted from control among all sets analyzed with respect to new and augmented, as well as suppressed and deleted polypeptides. Using hybridization analysis, there is preliminary evidence that cell-specific transcripts are augmented.
- Research Organization:
- Columbia Univ., New York (USA). Dept. of Pathology
- OSTI ID:
- 5377988
- Report Number(s):
- AD-A-188579/7/XAB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
PHYSICAL RADIATION EFFECTS
RNA
AUTORADIOGRAPHY
TRITIUM
TRACER TECHNIQUES
BIOCHEMISTRY
DROSOPHILA
ELECTROMAGNETIC FIELDS
GENES
HYBRIDIZATION
POLYMERS
POLYPEPTIDES
PROGRESS REPORT
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMISTRY
DIPTERA
DOCUMENT TYPES
FLIES
FRUIT FLIES
HYDROGEN ISOTOPES
INSECTS
INVERTEBRATES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PROTEINS
RADIATION EFFECTS
RADIOISOTOPES
YEARS LIVING RADIOISOTOPES
560400* - Other Environmental Pollutant Effects
550301 - Cytology- Tracer Techniques