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Title: Distribution of chromosome 18 and X centric heterochromatin in the interphase nucleus of cultured human cells

In situ hybridization of human chromosome 18 and X-specific alphoid DNA-probes was performed in combination with three dimensional (3D) and two dimensional (2D) image analysis to study the interphase distribution of the centric heterochromatin (18c and Xc) of these chromosomes in cultured human cells. 3D analyses of 18c targets using confocal laser scanning microscopy indicated a nonrandom disposition in 73 amniotic fluid cell nuclei. In agreement with the 3D observations 18c targets were found significantly closer to the center of the 2D nuclear image (CNI) and to each other in all these cultures compared to a random distribution derived from corresponding ellipsoid or cylinder model nuclei. For comparison, a chromosome X-specific alphoid DNA probe was used to investigate the 2D distribution of chromosome X centric heterochromatin in the same cell types. Two dimensional Xc-Xc and Xc-CNI distances fit a random distribution in diploid normal and Bloom's syndrome nuclei, as well as in nuclei with trisomy X. The different distributions of 18c and Xc targets were confirmed by the simultaneous staining of these targets in different colors within individual nuclei using a double in situ hybridization approach.
Authors:
; ; ; ; ;  [1] ;  [2]
  1. (Univ. of Heidelberg (West Germany))
  2. (European Molecular Biology Lab., Heidelberg (West Germany))
Publication Date:
OSTI Identifier:
5377114
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; (United States); Journal Volume: 189:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HETEROCHROMATIN; SUBCELLULAR DISTRIBUTION; HETEROCHROMOSOMES; DNA HYBRIDIZATION; HUMAN X CHROMOSOME; CELL CULTURES; CELL CYCLE; FIBROBLASTS; MAN; TWO-DIMENSIONAL ELECTROPHORESIS; ANIMAL CELLS; ANIMALS; CHROMATIN; CHROMOSOMES; CONNECTIVE TISSUE CELLS; DISTRIBUTION; ELECTROPHORESIS; HUMAN CHROMOSOMES; HYBRIDIZATION; MAMMALS; PRIMATES; SOMATIC CELLS; VERTEBRATES; X CHROMOSOME 550400* -- Genetics