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Title: Investigation of early molybdopterin biosynthetic intermediates

Abstract

Little information is available regarding the early steps in the biosynthetic pathway of molybdopterin (MPT). In order to explore these early reactions, and in particular to investigate the origin of the ring and side chain carbons of MPT, a metabolic approach employing the incorporation of {sup 14}C label was chosen. This method was facilitated by the recent purification and characterization of desulfomolybdopterin 2{prime},4{prime}-cyclic phosphate, the precursor which is converted directly to active molybdopterin in Escherichia coli by the addition of vicinal sulfurs to the side chain. This labile precursor readily oxidizes to Compound Z, a stable 6-alkyl pterin which retains all of the carbon atoms present in molybdopterin. Compound Z, rather than molybdopterin itself was chosen as the end product for labeling due to its overproduction in some MPT-deficient strains, as well as its stability and ease of purification. The authors report here the isolation of {sup 14}C-labelled Compound Z from E.coli chlN cells cultured in minimal media supplemented with U-{sup 14}C guanosine. Successive cleavage of the side chain carbons by permanganate treatment and UV light produced a decrease in the specific radioactivity of the resulting pterins. These data indicate that the early portion of the molybdopterin biosynthetic pathway maymore » be similar to that of the bioactive pterins folate and biopterin, both of which are derived from guanosine triphosphate.« less

Authors:
;  [1]
  1. Duke Univ., Durham, NC (United States)
Publication Date:
OSTI Identifier:
5374414
Report Number(s):
CONF-9104107-
Journal ID: ISSN 0892-6638; CODEN: FAJOE
Resource Type:
Conference
Journal Name:
FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
Additional Journal Information:
Journal Volume: 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; Journal ID: ISSN 0892-6638
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; METALLOPROTEINS; BIOSYNTHESIS; MOLYBDENUM; METABOLISM; BIOLOGICAL PATHWAYS; CARBON 14 COMPOUNDS; ESCHERICHIA COLI; TRACER TECHNIQUES; BACTERIA; CARBON COMPOUNDS; ELEMENTS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; METALS; MICROORGANISMS; ORGANIC COMPOUNDS; PROTEINS; SYNTHESIS; TRANSITION ELEMENTS; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Wuebbens, M M, and Rajagopalan, K V. Investigation of early molybdopterin biosynthetic intermediates. United States: N. p., 1991. Web.
Wuebbens, M M, & Rajagopalan, K V. Investigation of early molybdopterin biosynthetic intermediates. United States.
Wuebbens, M M, and Rajagopalan, K V. 1991. "Investigation of early molybdopterin biosynthetic intermediates". United States.
@article{osti_5374414,
title = {Investigation of early molybdopterin biosynthetic intermediates},
author = {Wuebbens, M M and Rajagopalan, K V},
abstractNote = {Little information is available regarding the early steps in the biosynthetic pathway of molybdopterin (MPT). In order to explore these early reactions, and in particular to investigate the origin of the ring and side chain carbons of MPT, a metabolic approach employing the incorporation of {sup 14}C label was chosen. This method was facilitated by the recent purification and characterization of desulfomolybdopterin 2{prime},4{prime}-cyclic phosphate, the precursor which is converted directly to active molybdopterin in Escherichia coli by the addition of vicinal sulfurs to the side chain. This labile precursor readily oxidizes to Compound Z, a stable 6-alkyl pterin which retains all of the carbon atoms present in molybdopterin. Compound Z, rather than molybdopterin itself was chosen as the end product for labeling due to its overproduction in some MPT-deficient strains, as well as its stability and ease of purification. The authors report here the isolation of {sup 14}C-labelled Compound Z from E.coli chlN cells cultured in minimal media supplemented with U-{sup 14}C guanosine. Successive cleavage of the side chain carbons by permanganate treatment and UV light produced a decrease in the specific radioactivity of the resulting pterins. These data indicate that the early portion of the molybdopterin biosynthetic pathway may be similar to that of the bioactive pterins folate and biopterin, both of which are derived from guanosine triphosphate.},
doi = {},
url = {https://www.osti.gov/biblio/5374414}, journal = {FASEB Journal (Federation of American Societies for Experimental Biology); (United States)},
issn = {0892-6638},
number = ,
volume = 5:4,
place = {United States},
year = {Mon Mar 11 00:00:00 EST 1991},
month = {Mon Mar 11 00:00:00 EST 1991}
}

Conference:
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