skip to main content

Title: RNA Polymerase III transcription in higher plants: Annual performance report, December 20, 1986 through December 15, 1987

tDNA-dependent Pol III transcription is not observed directly in extracts of wheat embryo cells or nuclei. This project seeks to purify wheat germ RNA Polymerase III, purify wheat factor TFIIIC based upon tDNA binding activity, and by using the two above components plus a specific cloned tDNA template, to screen extracts for the one missing component.
Authors:
Publication Date:
OSTI Identifier:
5359188
Report Number(s):
DOE/ER/13517-2
ON: DE88006906
DOE Contract Number:
FG06-86ER13517
Resource Type:
Technical Report
Research Org:
Washington Univ., Seattle (USA). Dept. of Genetics
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; DNA POLYMERASES; FRACTIONATION; ENZYME INHIBITORS; TRANSFER RNA; GENES; LIQUID COLUMN CHROMATOGRAPHY; PHOSPHORUS 32; PROGRESS REPORT; TRACER TECHNIQUES; WHEAT; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CEREALS; CHROMATOGRAPHY; DAYS LIVING RADIOISOTOPES; DOCUMENT TYPES; ENZYMES; GRASS; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; NUCLEI; NUCLEIC ACIDS; NUCLEOTIDYLTRANSFERASES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; PHOSPHORUS-GROUP TRANSFERASES; PLANTS; POLYMERASES; RADIOISOTOPES; RNA; SEPARATION PROCESSES; TRANSFERASES 550200* -- Biochemistry; 140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)