Isoelectric focusing purity criteria and /sup 1/H NMR detectable spectroscopic heterogeneity in the major isolated monomer hemoglobins from Glycera dibranchiata

Journal Article · Tue Dec 01 00:00:00 EST 1987 · Biochemistry; (United States)

OSTI ID:5354761

Constantinidis, I; Satterlee, J D

Three major monomeric hemoglobins have been isolated from the erythrocytes of Glycera dibranchiata. Their importance to structure-function studies of heme proteins lies in the fact that they have been shown to possess an exceptional amino acid substitution. In these proteins, the E-7 position is occupied by leucine rather than the more common distal histidine. This substitution alters the polarity of the heme ligand binding environment compared to myoglobin. Due to this, the G. dibranchiata monomer hemoglobins are attracting much attention. However, until now no purity criterion has been developed. Here the authors demonstrate that, for all of the Glycera momomer hemoglobins, multiple line patterns are shown on high-voltage isoelectric focusing (IEF) gels. Most of these lines are shown to be a consequence of heme-related phenomena and can be understood on the basis of changes in oxidation and ligation state of the heme iron. The multiple line pattern does not indicate significant impurities in the monomer hemoglobin preparation. The multiple line patterns on IEF gels disappear when gels of the apoproteins alone are focused. Single bands occur in this case for all of the monomer hemoglobins except component II, which displays two bands, one major and one minor. The minor band is found to be a modified apoprotein form. It is sensitive to apoprotein handling prior to focusing and depends upon whether the IEF gel is prefocused or not. From this analysis, IEF is shown to be a valuable purity criterion, and the purity of our monomer hemoglobin component II preparation is 97% one globin. The NMR results show that two types of spectroscopic heterogeneity are also present in component II, and these are unrelated to the protein purity.

• Research Organization: Univ. of New Mexico, Albuquerque
• OSTI ID: 5354761
• Journal Information: Biochemistry; (United States), Vol. 26:24
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
HEMOGLOBIN
ELECTROPHORESIS
NUCLEAR MAGNETIC RESONANCE
STRUCTURAL CHEMICAL ANALYSIS
ANNELIDS
ERYTHROCYTES
HEAVY WATER
HISTIDINE
HORSES
LEUCINE
MYOGLOBIN
PROTONS
AMINO ACIDS
ANIMALS
AZOLES
BARYONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
ELEMENTARY PARTICLES
FERMIONS
GLOBIN
HADRONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
IMIDAZOLES
INVERTEBRATES
MAGNETIC RESONANCE
MAMMALS
MATERIALS
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RESONANCE
VERTEBRATES
WATER
550601* - Medicine- Unsealed Radionuclides in Diagnostics