Application of the shock layer theory to the determination of the mass transfer rate coefficient and its concentration dependence for proteins on anion exchange columns
- Univ. of Tennessee, Knoxville, TN (United States)
The extension of the shock layer theory to systems having a slow mass transfer kinetics and a concentration-dependent rate coefficient is discussed. Experiments were carried out with bovine serum albumin on two anion exchanges, TSK-GEL-DEAE-5PW and Resource-Q. The adsorption isotherm data, determined by single-step frontal analysis, could be fitted to simplified bi-Langmuir equations with vary small residuals. A lumped kinetic model (solid film linear driving force model, with rate coefficient k{sub f}) was used to account for the mass transfer kinetics. The profile of each breakthrough curve (BC) was fitted to the curve calculated with this transport model and the rate coefficient k{sub f} obtained by identification. A linear dependence of k{sub f} on the average concentration of the step of the BC was found. The shock layer thicknesses (SLT) calculated for different relative concentrations agreed very well with the experimental results. This justifies the use of the SLT for the direct determination of rate coefficients. 19 refs., 9 figs., 2 tabs.
- OSTI ID:
- 535442
- Journal Information:
- Biotechnology Progress, Vol. 13, Issue 2; Other Information: PBD: Mar-Apr 1997
- Country of Publication:
- United States
- Language:
- English
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