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Title: Haplotype test reveals departure from neutrality in a segment of the white gene of Drosophila melanogaster

Abstract

Restriction map studies previously revealed extensive linkage disequilibria in the transcriptional unit of the white locus in natural Drosophila melanogaster populations. To understand the causes of these disequilibria, we sequenced a 4722-bp region of the white gene from 15 lines of D. melanogaster and 1 line of Drosophila simulans. Statistical tests applied to the entire 4722-bp region do not reject neutrality. In contrast, a test for high-frequency haplotypes ({open_quotes}Haplotype test{close_quotes}) revealed an 834-bp segment, encompassing the 3{prime} end of intron 1 to the 3{prime} end of intron 2, in which the structure of variation deviates significantly from the predictions of a neutral equilibrium model. The variants in this 834-bp segment segregate as single haplotype blocks. We propose that these unusually large haplotype blocks are due to positive selection on polymorphisms within the white gene, including a replacement polymorphism, Arg{yields}Leu, within this segment. 45 refs., 4 figs., 1 tab.

Authors:
;  [1]
  1. Univ. of Maryland, College Park, MD (United States)
Publication Date:
OSTI Identifier:
535349
Resource Type:
Journal Article
Journal Name:
Genetics
Additional Journal Information:
Journal Volume: 141; Journal Issue: 4; Other Information: PBD: Dec 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DROSOPHILA; POPULATIONS; GENETICS; GENES; GENETIC MAPPING; TRANSCRIPTION; MUTATION FREQUENCY; DNA SEQUENCING; STRUCTURE-ACTIVITY RELATIONSHIPS; GENE MUTATIONS; STATISTICS; INTRONS; AMINO ACIDS

Citation Formats

Kirby, D A, and Stephan, W. Haplotype test reveals departure from neutrality in a segment of the white gene of Drosophila melanogaster. United States: N. p., 1995. Web.
Kirby, D A, & Stephan, W. Haplotype test reveals departure from neutrality in a segment of the white gene of Drosophila melanogaster. United States.
Kirby, D A, and Stephan, W. 1995. "Haplotype test reveals departure from neutrality in a segment of the white gene of Drosophila melanogaster". United States.
@article{osti_535349,
title = {Haplotype test reveals departure from neutrality in a segment of the white gene of Drosophila melanogaster},
author = {Kirby, D A and Stephan, W},
abstractNote = {Restriction map studies previously revealed extensive linkage disequilibria in the transcriptional unit of the white locus in natural Drosophila melanogaster populations. To understand the causes of these disequilibria, we sequenced a 4722-bp region of the white gene from 15 lines of D. melanogaster and 1 line of Drosophila simulans. Statistical tests applied to the entire 4722-bp region do not reject neutrality. In contrast, a test for high-frequency haplotypes ({open_quotes}Haplotype test{close_quotes}) revealed an 834-bp segment, encompassing the 3{prime} end of intron 1 to the 3{prime} end of intron 2, in which the structure of variation deviates significantly from the predictions of a neutral equilibrium model. The variants in this 834-bp segment segregate as single haplotype blocks. We propose that these unusually large haplotype blocks are due to positive selection on polymorphisms within the white gene, including a replacement polymorphism, Arg{yields}Leu, within this segment. 45 refs., 4 figs., 1 tab.},
doi = {},
url = {https://www.osti.gov/biblio/535349}, journal = {Genetics},
number = 4,
volume = 141,
place = {United States},
year = {Fri Dec 01 00:00:00 EST 1995},
month = {Fri Dec 01 00:00:00 EST 1995}
}