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Title: Phytochrome regulation of gibberellin metabolism in shoots of dwarf Pisum sativum L

Abstract

To study the effect of light on the recessive dwarfing allele, le, of Pisum sativum L., etiolated, paclobutrazol treated LeLe (cv. Alaska) and lele (cv. Progress) pea seedlings were transferred to different light regimes. The growth response of both genotypes to applications of GA/sub 1/, GA/sub 20/, and steviol was measured over 48 hours using position transducers. Both genotypes responded to GA/sub 1/ under red irradiation and in darkness. The LeLe plants grew in response to steviol and GA/sub 20/ under red irradiation and in darkness. The lele plants responded to steviol and GA/sub 20/ in darkness, but showed a much smaller response when red irradiated. The red effect on lele plants was reversible by far-red irradiation. (/sup 3/H)GA/sub 20/ was applied to nana pea seedlings, homozygous for le, grown under different light regimes. Radioactive metabolites were later extracted from the shoots of the treated plants. Both the free acid and conjugate pools were analyzed by reversed phase HPLC, and some radioactive metabolites were tentatively identified by comparing their retention times to those of authentic (/sup 3/H)GAs.

Authors:
Publication Date:
Research Org.:
California Univ., Davis (USA)
OSTI Identifier:
5347711
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GIBBERELLIC ACID; METABOLISM; METABOLITES; RETENTION; LIQUID COLUMN CHROMATOGRAPHY; PISUM; SEEDLINGS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; VISIBLE RADIATION; BACTERIA; CARBOXYLIC ACIDS; CHROMATOGRAPHY; ELECTROMAGNETIC RADIATION; ESTERS; HETEROCYCLIC COMPOUNDS; HYDROXY ACIDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LACTONES; LEGUMINOSAE; MICROORGANISMS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PLANTS; RADIATIONS; RHIZOBIUM; SEPARATION PROCESSES; 550501* - Metabolism- Tracer Techniques

Citation Formats

Campell, B R. Phytochrome regulation of gibberellin metabolism in shoots of dwarf Pisum sativum L. United States: N. p., 1986. Web.
Campell, B R. Phytochrome regulation of gibberellin metabolism in shoots of dwarf Pisum sativum L. United States.
Campell, B R. 1986. "Phytochrome regulation of gibberellin metabolism in shoots of dwarf Pisum sativum L". United States.
@article{osti_5347711,
title = {Phytochrome regulation of gibberellin metabolism in shoots of dwarf Pisum sativum L},
author = {Campell, B R},
abstractNote = {To study the effect of light on the recessive dwarfing allele, le, of Pisum sativum L., etiolated, paclobutrazol treated LeLe (cv. Alaska) and lele (cv. Progress) pea seedlings were transferred to different light regimes. The growth response of both genotypes to applications of GA/sub 1/, GA/sub 20/, and steviol was measured over 48 hours using position transducers. Both genotypes responded to GA/sub 1/ under red irradiation and in darkness. The LeLe plants grew in response to steviol and GA/sub 20/ under red irradiation and in darkness. The lele plants responded to steviol and GA/sub 20/ in darkness, but showed a much smaller response when red irradiated. The red effect on lele plants was reversible by far-red irradiation. (/sup 3/H)GA/sub 20/ was applied to nana pea seedlings, homozygous for le, grown under different light regimes. Radioactive metabolites were later extracted from the shoots of the treated plants. Both the free acid and conjugate pools were analyzed by reversed phase HPLC, and some radioactive metabolites were tentatively identified by comparing their retention times to those of authentic (/sup 3/H)GAs.},
doi = {},
url = {https://www.osti.gov/biblio/5347711}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1986},
month = {Wed Jan 01 00:00:00 EST 1986}
}

Thesis/Dissertation:
Other availability
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