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Title: New melanogenesis and photobiological processes in activation and proliferation of precursor melanocytes after UV-exposure: ultrastructural differentiation of precursor melanocytes from Langerhans cells

Abstract

Photobiological processes involving new melanogenesis after exposure to ultraviolet (UV) light were experimentally studied in C57 black adult mice by histochemistry, cytochemistry, and autoradiography. The trunk and the plantar region of the foot, where no functioning melanocytes were present before exposure, were exposed to UV-A for 14 consecutive days. Both regions revealed a basically similar pattern for new melanogenesis which involved an activation of precursor melanocytes. Essentially all of ''indeterminate'' cells appeared to be precursor melanocytes, the fine structure of which could be differentiated even from poorly developed Langerhans cells. New melanogenesis was manifested by 4 stages of cellular and subcellular reactions of these cells as indicated by histochemistry of dihydroxyphenylalanine (dopa) and autoradiography of thymidine incorporation: (a) an initial lag in the activation of precursor melanocytes with development of Golgi cisternae and rough endoplasmic reticulum followed by formation of unmelanized melanosomes (day 0 to 2); (b) synthesis of active tyrosinase accumulated in Golgi cisternae and vesicles with subsequent formation of melanized melanosomes in these cells (day 3 to 5); (c) mitotic proliferation of many of these activated cells, followed by an exponential increase of new melanocytes (day 6 to 7); and (d) melanosome transfer with differentiation of 10 nmmore » filaments and arborization of dendrites, but without any significant change in the melanocyte population (day 8 to 14). The melanosome transfer was, however, not obvious until after 7 days of exposure. The size of newly synthesized melanosomes was similar to that of tail skin where native melanocytes were present before exposure.« less

Authors:
;
Publication Date:
Research Org.:
Department of Dermatology, Sapporo Medical College, Japan
OSTI Identifier:
5343365
Resource Type:
Journal Article
Journal Name:
J. Invest. Dermatol.; (United States)
Additional Journal Information:
Journal Volume: 78:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANIMAL CELLS; BIOLOGICAL RADIATION EFFECTS; CELL DIFFERENTIATION; RADIOINDUCTION; CELL PROLIFERATION; MELANIN; AUTORADIOGRAPHY; CYTOCHEMISTRY; DOPA; MICE; NEAR ULTRAVIOLET RADIATION; AMINO ACIDS; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; BIOCHEMISTRY; BIOLOGICAL EFFECTS; CARBOXYLIC ACIDS; CHEMISTRY; DRUGS; ELECTROMAGNETIC RADIATION; HYDROXY ACIDS; HYDROXY COMPOUNDS; MAMMALS; NEUROREGULATORS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PIGMENTS; RADIATION EFFECTS; RADIATIONS; RODENTS; ULTRAVIOLET RADIATION; VERTEBRATES; 560121* - Radiation Effects on Cells- External Source- (-1987); 560152 - Radiation Effects on Animals- Animals

Citation Formats

Jimbow, K, and Uesugi, T. New melanogenesis and photobiological processes in activation and proliferation of precursor melanocytes after UV-exposure: ultrastructural differentiation of precursor melanocytes from Langerhans cells. United States: N. p., 1982. Web. doi:10.1111/1523-1747.ep12505758.
Jimbow, K, & Uesugi, T. New melanogenesis and photobiological processes in activation and proliferation of precursor melanocytes after UV-exposure: ultrastructural differentiation of precursor melanocytes from Langerhans cells. United States. https://doi.org/10.1111/1523-1747.ep12505758
Jimbow, K, and Uesugi, T. 1982. "New melanogenesis and photobiological processes in activation and proliferation of precursor melanocytes after UV-exposure: ultrastructural differentiation of precursor melanocytes from Langerhans cells". United States. https://doi.org/10.1111/1523-1747.ep12505758.
@article{osti_5343365,
title = {New melanogenesis and photobiological processes in activation and proliferation of precursor melanocytes after UV-exposure: ultrastructural differentiation of precursor melanocytes from Langerhans cells},
author = {Jimbow, K and Uesugi, T},
abstractNote = {Photobiological processes involving new melanogenesis after exposure to ultraviolet (UV) light were experimentally studied in C57 black adult mice by histochemistry, cytochemistry, and autoradiography. The trunk and the plantar region of the foot, where no functioning melanocytes were present before exposure, were exposed to UV-A for 14 consecutive days. Both regions revealed a basically similar pattern for new melanogenesis which involved an activation of precursor melanocytes. Essentially all of ''indeterminate'' cells appeared to be precursor melanocytes, the fine structure of which could be differentiated even from poorly developed Langerhans cells. New melanogenesis was manifested by 4 stages of cellular and subcellular reactions of these cells as indicated by histochemistry of dihydroxyphenylalanine (dopa) and autoradiography of thymidine incorporation: (a) an initial lag in the activation of precursor melanocytes with development of Golgi cisternae and rough endoplasmic reticulum followed by formation of unmelanized melanosomes (day 0 to 2); (b) synthesis of active tyrosinase accumulated in Golgi cisternae and vesicles with subsequent formation of melanized melanosomes in these cells (day 3 to 5); (c) mitotic proliferation of many of these activated cells, followed by an exponential increase of new melanocytes (day 6 to 7); and (d) melanosome transfer with differentiation of 10 nm filaments and arborization of dendrites, but without any significant change in the melanocyte population (day 8 to 14). The melanosome transfer was, however, not obvious until after 7 days of exposure. The size of newly synthesized melanosomes was similar to that of tail skin where native melanocytes were present before exposure.},
doi = {10.1111/1523-1747.ep12505758},
url = {https://www.osti.gov/biblio/5343365}, journal = {J. Invest. Dermatol.; (United States)},
number = ,
volume = 78:2,
place = {United States},
year = {Mon Feb 01 00:00:00 EST 1982},
month = {Mon Feb 01 00:00:00 EST 1982}
}