Effect of dietary fibers on cholic acid induced cell proliferation in the colonic epithelium of C57BL/6J mice
It has been postulated that high fat diets promote tumorigenesis by increasing the level of secondary bile acids in the colonic lumen. Dietary fibers are thought to be protective perhaps through their interaction with bile acids. In the present study, animals were fed diets containing either 0%, 5%, or 10% cellulose (C), pectin (P), or wheat bran (WB). The diets were formulated to contain either 0% (control) or 0.2% cholic acid (test). After two weeks of dietary treatment the animals were injected with (/sup 3/H)-thymidine and their colons were processed for autoradiography. The number of labeled cells (LC) in the colonic crypts was determined. Among the control diets, 10%P induced a two-fold increase in the LC. All the test groups had significantly higher LC than in their controls. However, the C group excited a higher LC than the P or WB groups (5.2 +/- 0.8 vs 3.9 +/- 0.8 or 3.9 +/- 0.6). These results were substantiated by metaphase arrest technique. The authors results show that nonfermentable fiber does not alleviate bile acid induced cell proliferative activity in the colon whereas fermentable fibers will counteract the promotional effect of a high fat diet.
- Research Organization:
- Univ. of Toronto, Ontario
- OSTI ID:
- 5332185
- Report Number(s):
- CONF-8604222-; TRN: 86-031458
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BILE ACIDS
CARCINOGENESIS
CRYPT CELLS
CELL PROLIFERATION
FIBERS
BIOLOGICAL EFFECTS
AUTORADIOGRAPHY
CELLULOSE
CHOLIC ACID
DIET
LARGE INTESTINE
MICE
PECTINS
THYMIDINE
TRITIUM COMPOUNDS
WHEAT
ANIMAL CELLS
ANIMALS
AZINES
BLOOD SUBSTITUTES
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CEREALS
DIGESTIVE SYSTEM
DRUGS
GASTROINTESTINAL TRACT
GRASS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INTESTINES
LABELLED COMPOUNDS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOGENESIS
PLANTS
POLYSACCHARIDES
PYRIMIDINES
RIBOSIDES
RODENTS
SACCHARIDES
SOMATIC CELLS
STEROIDS
STEROLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques