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Title: Generation of fatty acids by an acyl esterase in the bioluminescent system of Photobacterium phosphoreum

Abstract

The fatty acid reductase complex from Photobacterium phosphoreum has been discovered to have a long chain ester hydrolase activity associated with the 34K protein component of the complex. This protein has been resolved from the other components (50K and 58K) of the fatty acid reductase complex with a purity of > 95% and found to catalyze the transfer of acyl groups from acyl-CoA primarily to thiol acceptors with a low level of transfer to glycerol and water. Addition of the 50K protein of the complex caused a dramatic change in specificity increasing the transfer to oxygen acceptors. The acyl-CoA hydrolase activity increased almost 10-fold, and hence free fatty acids can be generated by the 34K protein when it is present in the fatty acid reductase complex. Hydrolysis of acyl-S-mercaptoethanol and acyl-1-glycerol and the ATP-dependent reduction of the released fatty acids to aldehyde for the luminescent reaction were also demonstrated for the reconstituted fatty acid reductase complex, raising the possibility that the immediate source of fatty acids for this reaction in vivo could be the membrane lipids and/or the fatty acid synthetase system.

Authors:
; ;
Publication Date:
Research Org.:
McGill Univ., Montreal, Quebec
OSTI Identifier:
5332029
Resource Type:
Journal Article
Journal Name:
J. Biol. Chem.; (United States)
Additional Journal Information:
Journal Volume: 259:16
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BACTERIA; ENZYME ACTIVITY; CARBOXYLIC ACIDS; BIOSYNTHESIS; ESTERASES; BIOCHEMISTRY; BIOLUMINESCENCE; CELL MEMBRANES; ELECTROPHORESIS; EXTREME ULTRAVIOLET RADIATION; PHOTOCHEMISTRY; CELL CONSTITUENTS; CHEMISTRY; ELECTROMAGNETIC RADIATION; ENZYMES; HYDROLASES; LUMINESCENCE; MEMBRANES; MICROORGANISMS; ORGANIC ACIDS; ORGANIC COMPOUNDS; RADIATIONS; SYNTHESIS; ULTRAVIOLET RADIATION; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Carey, L M, Rodriguez, A, and Meighen, E. Generation of fatty acids by an acyl esterase in the bioluminescent system of Photobacterium phosphoreum. United States: N. p., 1984. Web.
Carey, L M, Rodriguez, A, & Meighen, E. Generation of fatty acids by an acyl esterase in the bioluminescent system of Photobacterium phosphoreum. United States.
Carey, L M, Rodriguez, A, and Meighen, E. 1984. "Generation of fatty acids by an acyl esterase in the bioluminescent system of Photobacterium phosphoreum". United States.
@article{osti_5332029,
title = {Generation of fatty acids by an acyl esterase in the bioluminescent system of Photobacterium phosphoreum},
author = {Carey, L M and Rodriguez, A and Meighen, E},
abstractNote = {The fatty acid reductase complex from Photobacterium phosphoreum has been discovered to have a long chain ester hydrolase activity associated with the 34K protein component of the complex. This protein has been resolved from the other components (50K and 58K) of the fatty acid reductase complex with a purity of > 95% and found to catalyze the transfer of acyl groups from acyl-CoA primarily to thiol acceptors with a low level of transfer to glycerol and water. Addition of the 50K protein of the complex caused a dramatic change in specificity increasing the transfer to oxygen acceptors. The acyl-CoA hydrolase activity increased almost 10-fold, and hence free fatty acids can be generated by the 34K protein when it is present in the fatty acid reductase complex. Hydrolysis of acyl-S-mercaptoethanol and acyl-1-glycerol and the ATP-dependent reduction of the released fatty acids to aldehyde for the luminescent reaction were also demonstrated for the reconstituted fatty acid reductase complex, raising the possibility that the immediate source of fatty acids for this reaction in vivo could be the membrane lipids and/or the fatty acid synthetase system.},
doi = {},
url = {https://www.osti.gov/biblio/5332029}, journal = {J. Biol. Chem.; (United States)},
number = ,
volume = 259:16,
place = {United States},
year = {Sat Aug 25 00:00:00 EDT 1984},
month = {Sat Aug 25 00:00:00 EDT 1984}
}