Species differences in biliary excretion of benzo(a)pyrene
Biliary excretion of benzo(a)pyrene (B(a)P) was investigated in rats, hamsters, and guinea pigs following intratracheal administration. (/sup 3/H)-B(a)P, in amounts of approximately 150 ng or 350 ..mu..g, was instilled into lungs and amounts of radioactivity excreted in bile were monitored for six hrs following administration. Differences in biliary excretion of (/sup 3/H)-B(a)P and/or metabolites among species were observed at low doses but not at high doses. Six hours after instillation of a low dose of B(a)P, 70, 54, and 62% of the dose was excreted in bile of rats, hamsters, and guinea pigs, respectively. Upon administration of the higher dose of B(a)P, approximately 50% of the dose was excreted in bile in six hrs by all species. Thus, rats and guinea pigs exhibit differences in biliary excretion of low and high doses of B(a)P whereas hamsters do not. Profiles of phase II metabolites in rats and hamsters were similar at both low and high doses, with the majority of metabolites being glucuronides and thioether conjugates. However, differences in relative amounts of these conjugates were observed between the two doses, with a shift towards a greater proportion of glucuronides at the higher dose. Metabolites in bile from guinea pigs were primarily thioether conjugates, which accounted for 88% of metabolites at the low dose and 95% at the high dose.
- Research Organization:
- Virginia Polytechnic Institute and State Univ., Blacksburg
- OSTI ID:
- 5331643
- Report Number(s):
- CONF-8606151-; TRN: 86-031481
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BENZOPYRENE
EXCRETION
METABOLISM
BIOLOGICAL VARIABILITY
FECES
GUINEA PIGS
HAMSTERS
INTRATRACHEAL ADMINISTRATION
LUNGS
METABOLITES
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
AROMATICS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
CLEARANCE
CONDENSED AROMATICS
HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANS
RESPIRATORY SYSTEM
RODENTS
VERTEBRATES
WASTES
550501* - Metabolism- Tracer Techniques