Heparin fragments inhibit human vascular smooth muscle cell proliferation in vitro

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5326403

Selden, S C; Johnson, W V; Maciag, T

The authors have examined the effect of heparin on human abdominal aortic smooth muscle cell growth. Cell proliferation was inhibited by more than 90% at a concentration of 20 ..mu..g/ml in a 12 day growth assay using heparin from Sigma, Upjohn or Calbiochem. Additionally, 200 ..mu..g/ml Upjohn heparin inhibits /sup 3/H-thymidine incorporation by 50% in short term assays using serum or purified platelet-derived growth factor (25-100ng/ml) to initiate the cell cycle. Homogeneous size classes of heparin fragments were prepared by nitrous acid cleavage and BioGel P-10 filtration chromatography. Deca-, octa-, hexa-, tetra-, and di-saccharides inhibited proliferation by 90% at concentrations of 280, 320, 260, 180 and 100 ..mu..g/ml, respectively, in a 12 day growth assay. These data confirm the work of Castellot et.al. and extend the range of inhibitory fragments down to the tetra- and di-saccharide size. These data suggest, therefore, that di-saccharide subunit of heparin is sufficient to inhibit vascular smooth muscle cell proliferation. The authors are now examining the role of the anhydromannose moiety on the reducing end of the nitrous acid generated fragments as a possible mediator of heparin-induced inhibition of vascular smooth muscle cell proliferation.

• Research Organization: Biotechnology Research Center, Rockville, MD
• OSTI ID: 5326403
• Report Number(s): CONF-8604222-; TRN: 86-030435
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HEPARIN
BIOLOGICAL EFFECTS
MUSCLES
CELL PROLIFERATION
ABDOMEN
CHROMATOGRAPHY
IN VITRO
INHIBITION
MANNOSE
SACCHARIDES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALDEHYDES
AMINES
ANTICOAGULANTS
BODY
BODY AREAS
CARBOHYDRATES
DRUGS
HEMATOLOGIC AGENTS
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MONOSACCHARIDES
MUCOPOLYSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYSACCHARIDES
SEPARATION PROCESSES
550201* - Biochemistry- Tracer Techniques