Molecular cloning of cDNA for the human tumor-associated antigen CO-029 and identification of related transmembrane antigens

Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.17.6833· OSTI ID:5323843

Szala, S; Kasai, Yasushi; Steplewski, Z; Rodeck, U; Koprowski, H; Linnenbach, A J ^[1]

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1. Wistar Inst. of Anatomy and Biology, Philadelphia, PA (USA)

The human tumor-associated antigen CO-029 is a monoclonal antibody-defined cell surface glycoprotein of 27-34 kDa. By using the high-efficiency COS cell expression system, a full-length cDNA clone for CO-029 was isolated. When transiently expressed in COS cells, the cDNA clone directed the synthesis of an antigen reactive to monoclonal antibody CO-029 in mixed hemadsorption and immunoblot assays. Sequence analysis revealed that CO-029 belongs to a family of cell surface antigens that includes the melanoma-associated antigen ME491, the leukocyte cell surface antigen CD37, and the Sm23 antigen of the parasitic helminth Schistosoma mansoni. CO-029 and ME491 antigen expression and the effect of their corresponding monoclonal antibodies on cell growth were compared in human tumor cell lines of various histologic origins.

• OSTI ID: 5323843
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 87:17; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIGENS
BIOSYNTHESIS
GLYCOPROTEINS
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA-CLONING
DNA SEQUENCING
ESCHERICHIA COLI
IODINE 125
LEUKOCYTES
MAN
MONOCLONAL ANTIBODIES
NEOPLASMS
TUMOR CELLS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CLONING
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
DNA HYBRIDIZATION
ELECTRON CAPTURE RADIOISOTOPES
HYBRIDIZATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE ISOTOPES
ISOTOPES
MAMMALS
MATERIALS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques