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Title: Stromal cell migration precedes hemopoietic repopulation of the bone marrow after irradiation

Circulation of hemopoietic stem cells into an irradiated site has been thoroughly documented, but migration of stromal cells to repair radiation damage has not. We determined the radiosensitivity of mouse bone marrow stroma and evaluated stromal and hemopoietic repopulation in x-irradiated marrow. The D/sub 0/ for growth of colonies of marrow stromal cells (MSC) was 215 to 230 rad. Total-body irradiation (TB) obliterated marrow stromal and hemopoietic cells within 3 days. In contrast, 1 day after 1000 rad leg irradiation (LI), MSC rose to 80% of normal, but fell to 34% by 3 days and recovered to 72% by 30 days. However, femoral nucleated cells diminished to 20% by 3 days and recovered to 74% of normal by 30 days. Likewise, differentiated marrow cells and hemopoietic stem cells were initially depleted. With 1000 rad LI followed 3 h later by 1000 rad to the body while shielding the leg, MSC and femoral nucleated cells recovered to values intermediate between 1000 rad TB and 1000 rad LI. We concluded that: (1) the D/sub 0/ for MSC was 215 to 230 rad, (2) stromal repopulation preceded hemopoietic recovery, and (3) immigration of stromal cells from an unirradiated sanctuary facilitated hemopoietic repopulation ofmore » a heavily irradiated site.« less
Authors:
; ; ;
Publication Date:
OSTI Identifier:
5323153
Resource Type:
Journal Article
Resource Relation:
Journal Name: Radiat. Res.; (United States); Journal Volume: 81:1
Research Org:
Univ. of Iowa, Iowa City
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; BONE MARROW; RADIOSENSITIVITY; BONE MARROW CELLS; X RADIATION; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; BLOOD CIRCULATION; COLONY FORMATION; COMPARATIVE EVALUATIONS; DOSE-RESPONSE RELATIONSHIPS; EXPERIMENTAL DATA; ISOLATED VALUES; MICE; MIGRATION; PARTIAL BODY IRRADIATION; STEM CELLS; SURVIVAL CURVES; WHOLE-BODY IRRADIATION; ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BODY; CONNECTIVE TISSUE CELLS; DATA; DATA FORMS; ELECTROMAGNETIC RADIATION; EXTERNAL IRRADIATION; HEMATOPOIETIC SYSTEM; INFORMATION; IONIZING RADIATIONS; IRRADIATION; MAMMALS; NUMERICAL DATA; ORGANS; RADIATION EFFECTS; RADIATIONS; RECOVERY; RODENTS; SOMATIC CELLS; TISSUES; VERTEBRATES 560121* -- Radiation Effects on Cells-- External Source-- (-1987); 560152 -- Radiation Effects on Animals-- Animals; 551000 -- Physiological Systems