Flow karyotyping and sorting of human chromosomes
Flow cytometry and sorting are becoming increasingly useful as tools for chromosome classfication and for the detection of numerical and structural chromosome aberrations. Chromosomes of a single type can be purified with these tools to facilitate gene mapping or production of chromosome specific recombinant DNA libraries. For analysis of chromosomes with flow cytometry, the chromosomes are extracted from mitotic cells, stained with one or more fluorescent dyes and classified one-by-one according to their dye content(s). Thus, the flow approach is fundamentally different than conventional karyotyping where chromosomes are classified within the context of a metaphase spread. Flow sorting allows purification of chromosomes that can be distinguished flow cytometrically. The authors describe the basic principles of flow cytometric chromosome classification i.e. flow karyotyping, and chromosome sorting and describe several applications. 30 refs., 8 figs.
- Research Organization:
- Lawrence Livermore National Lab., CA (USA). Biomedical Sciences Div.
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 5321598
- Report Number(s):
- UCRL-94969; CONF-8604241-1; ON: DE86014780
- Resource Relation:
- Conference: Cold Spring Harbor Symposia on quantitative biology: molecular biology of homo sapiens, Cold Spring Harbor, NY, USA, 20 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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