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Title: Radiation-induced hemopoietic and immune dysfunction. Technical report, 16 Aug 86-31 Mar 90

This work was aimed at investigating radiation-induced hemopoietic and immune dysfunction in the dog model. One project was to produce monoclonal antibodies directed against canine hemopoietic precursor cells and specific for pluripotent stem cells. Antibodies obtained reacted with different myeloid and erythroid precursor cells; unfortunately, none of the antibodies were specific for pluripotent hemopoietic stem cells. Positive selections for these cells were performed using magnetic beads and an antibody against class 2-antigen. Transplantation of class 2 positive marrow cells into otherwise lethally irradiated dogs led to sustained recovery in only 1 of 5 dogs. A second project established and investigated long-term marrow cultures in the dog. Culture conditions were studied and optimized, and marrow cells were transplanted into otherwise lethally irradiated dogs to investigate stem cell survival in long-term cultures. Engraftment was observed only with short-term marrow cultures.
Authors:
;
Publication Date:
OSTI Identifier:
5321268
Report Number(s):
AD-A-237256/3/XAB
CNN: DNA001-86-C-0192
Resource Type:
Technical Report
Research Org:
Fred Hutchinson Cancer Research Center, Seattle, WA (United States)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BONE MARROW CELLS; TRANSPLANTS; IMMUNE SYSTEM DISEASES; RADIOINDUCTION; STEM CELLS; SURVIVAL TIME; DOGS; ERYTHROCYTES; IMMUNITY; LETHAL IRRADIATION; MONOCLONAL ANTIBODIES; ANIMAL CELLS; ANIMALS; ANTIBODIES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DISEASES; IRRADIATION; MAMMALS; MATERIALS; SOMATIC CELLS; VERTEBRATES 560152* -- Radiation Effects on Animals-- Animals