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Title: Vasoactive intestinal peptide synergistically stimulates DNA synthesis in mouse 3T3 cells: Role of cAMP, Ca sup 2+ , and protein kinase C

Vasoactive intestinal peptide synergistically stimulated initiation of DNA synthesis in Swiss 3T3 cells. The peptide stimulated ({sup 3}H)thymidine incorporation in the presence of insulin and either forskolin or an inhibitor of cAMP phosphodiesterase in a concentration-dependent manner. Half-maximal effect was obtained at 1 nM. At mitogenic concentrations, VIP stimulated a marked accumulation (eightfold) of cAMP. In contrast to other growth-promoting neuropeptides, VIP did not induce an increase in cytosolic free Ca{sup 2+} or the activation of protein kinase C. The authors conclude that neuropeptides can modulate long-term cell proliferation through multiple signaling pathways.
Authors:
; ; ;  [1]
  1. (Imperial Cancer Research Fund, London (England))
Publication Date:
OSTI Identifier:
5321120
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; (United States); Journal Volume: 175:2
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; AMP; RADIOIMMUNOASSAY; FIBROBLASTS; DNA REPLICATION; GROWTH FACTORS; RADIORECEPTOR ASSAY; PEPTIDES; BIOCHEMISTRY; BIOCHEMICAL REACTION KINETICS; CALCIUM COMPOUNDS; CATIONS; INSULIN; IODINE 125; MICE; PHOSPHOTRANSFERASES; SYNERGISM; THYMIDINE; TRITIUM COMPOUNDS; ALKALINE EARTH METAL COMPOUNDS; ANIMAL CELLS; ANIMALS; AZINES; BETA DECAY RADIOISOTOPES; BIOASSAY; CHARGED PARTICLES; CHEMISTRY; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; HETEROCYCLIC COMPOUNDS; HORMONES; HYDROGEN COMPOUNDS; IMMUNOASSAY; IMMUNOLOGY; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; IONS; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MAMMALS; MITOGENS; NUCLEI; NUCLEIC ACID REPLICATION; NUCLEOSIDES; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PEPTIDE HORMONES; PHOSPHORUS-GROUP TRANSFERASES; PROTEINS; PYRIMIDINES; RADIOASSAY; RADIOIMMUNODETECTION; RADIOIMMUNOLOGY; RADIOISOTOPES; REACTION KINETICS; RIBOSIDES; RODENTS; SOMATIC CELLS; TRACER TECHNIQUES; TRANSFERASES; VERTEBRATES 550601* -- Medicine-- Unsealed Radionuclides in Diagnostics