Characterization of deoxyguanosine adducts from hydroquinone/benzoquinone
Occupational exposure to benzene has long been associated with the development of pancytopenia and leukemia. This toxicity has been attributed to the action of benzene metabolites. The authors have chosen to investigate the reaction of hydroquinone (HQ)/benzoquinone(BQ) with deoxyguanosine(dG) and DNA. (/sup 14/C)HQ was incubated with (/sup 3/H)dG in potassium phosphate buffer pH7.2 for 24 hours. Two dual labeled products were found by HPLC and presumed to be adducts. The same result was obtained when BQ was substituted in the reaction for HQ. Both adducts were found in isolated DNA from Clostridium perfringens, Micrococcus lysodeikticus, human placenta and calf thymus reacted with HO under similar conditions. One of the dG adducts was proposed to be (/sup 3/'OH) benzetheno(1,N-2)deoxyguanosine based on NMR and mass spectral results. The other adduct was characterized by a molecular weight of 339. The latter adduct was found in greater amounts than the former when HQ was reacted with denatured DNA.
- Research Organization:
- Rutgers, The State Univ. of NJ, Piscataway
- OSTI ID:
- 5316981
- Report Number(s):
- CONF-8604222-; TRN: 86-028573
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BENZOQUINONES
BIOLOGICAL EFFECTS
GUANOSINE
ADDUCTS
CALVES
CARBON 14 COMPOUNDS
CLOSTRIDIUM PERFRINGENS
DNA
LIQUID COLUMN CHROMATOGRAPHY
MAN
MASS SPECTROSCOPY
MICROCOCCUS LYSODEICTICUS
MOLECULAR WEIGHT
NMR SPECTRA
PLACENTA
QUINONES
THYMUS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
AROMATICS
AZAARENES
BACTERIA
BODY
CATTLE
CHROMATOGRAPHY
CLOSTRIDIUM
DOMESTIC ANIMALS
FETAL MEMBRANES
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYMPHATIC SYSTEM
MAMMALS
MEMBRANES
MICROCOCCUS
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PRIMATES
PURINES
RIBOSIDES
RUMINANTS
SEPARATION PROCESSES
SPECTRA
SPECTROSCOPY
VERTEBRATES
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)
560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)
550501 - Metabolism- Tracer Techniques