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Title: cap alpha. -chain locus of the T-cell antigen receptor is involved in the t(10; 14) chromosome translocation of T-cell acute lymphocytic leukemia

Human leukemic T cells carrying a t(10;14)(q24;q11) chromosome translocation were fused with mouse leukemic T cells, and the hybrids were examined for genetic markers of human chromosomes 10 and 14. Hybrids containing the human 10q+ chromosome had the human genes for terminal deoxynucleotidyltransferase that has been mapped at 10q23-q25 and for C/sub ..cap alpha../ (the constant region of TCRA (the ..cap alpha..-chain locus of the T-cell antigen receptor gene)), but not for V/sub ..cap alpha../ (the variable region of TCRA). Hybrids containing the human 14q- chromosome retained the V/sub ..cap alpha../genes. Thus the 14q11 breakpoint in the t(10;14) chromosome translocation directly involves TCRA, splitting the locus in a region between the V/sub ..cap alpha../ and the C/sub ..cap alpha../ genes. These results suggest that the translocation of the C/sub ..cap alpha../ locus to a putative cellular protooncogene located proximal to the breakpoint at 10q24, for which the authors propose the name TCL3, results in its deregulation, leading to T-cell leukemia. Since hybrids with the 10q+ chromosome also retained the human terminal deoxynucleotidyltransferase gene, it is further concluded that the terminal deoxynucleotidyltransferase locus is proximal to the TCL3 gene, at band 10q23-q24.
Authors:
; ; ; ; ;
Publication Date:
OSTI Identifier:
5316254
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States); Journal Volume: 84:13
Research Org:
Wistar Institute, Philadelphia, PA (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; LEUKEMIA; GENETICS; RECEPTORS; BIOCHEMISTRY; TUMOR CELLS; CHROMOSOMAL ABERRATIONS; HYBRIDIZATION; ANTIGENS; ELECTROPHORESIS; GENE RECOMBINATION; KARYOTYPE; LYMPHOCYTES; MICE; ONCOGENES; PATIENTS; PHOSPHORUS 32; TRANSFERASES; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BIOLOGY; BLOOD; BLOOD CELLS; BODY FLUIDS; CHEMISTRY; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DISEASES; ENZYMES; GENES; HEMIC DISEASES; ISOTOPES; LEUKOCYTES; LIGHT NUCLEI; MAMMALS; MATERIALS; MEMBRANE PROTEINS; MUTATIONS; NEOPLASMS; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; PROTEINS; RADIOISOTOPES; RODENTS; SOMATIC CELLS; VERTEBRATES 550401* -- Genetics-- Tracer Techniques; 550201 -- Biochemistry-- Tracer Techniques