Preparation and separation of the glucuronide and sulfate conjugates of thyroxine and triiodothyronine

Journal Article · Thu Jan 01 00:00:00 EST 1987 · Endocr. Res.; (United States)

DOI:https://doi.org/10.1080/07435808709035454· OSTI ID:5302797

Hays, M T; Hsu, L

An enzymatic method for synthesis of labelled thyroxine glucuronide (T4G) and triiodothyronine glucuronide (T3G) from labelled thyroxine (T4) and triiodothyronine (T3) is presented. The synthetic glucuronides are completely digested by beta-glucuronidase, with recovery of the parent T4 or T3. They have distinctive elution patterns on HPLC and on Sephadex G25 chromatography, and can be clearly separated from T4 and T3 as well as from synthetic T4 sulfate (T4S) and T3 sulfate (T3S). On LH 20 chromatography, elution of T4G and T3G is intermediate between that of T4 and T3 and that of T4S and T3S. T3G can be well separated from other thyronines by HPLC alone, but T4G coelutes with rT3 on HPLC; these are then separated by adding a Sephadex G25 chromatography step. Biosynthetic /sup 131/I-T3G and /sup 125/I-T4G from the bile of a cat given /sup 131/I-T3 and /sup 125/I-T4 had similar HPLC chromatographic patterns to those of synthetic T3G and T4G. That the identified peaks from analysis of the bile were indeed T3G and T4G was confirmed by recovery of the parent T3 and T4 after beta-glucuronidase digestion.

• Research Organization: Veterans Administration Medical Center, Palo Alto, CA (USA)
• OSTI ID: 5302797
• Journal Information: Endocr. Res.; (United States), Vol. 13:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCURONIDASE
ENZYME ACTIVITY
GLUCURONIDE CONJUGATES
CHEMICAL PREPARATION
FRACTIONATION
CATS
IN VITRO
IODINE 125
IODINE 131
LIQUID COLUMN CHROMATOGRAPHY
SULFATES
THYROXINE
TRACER TECHNIQUES
TRIIODOTHYRONINE
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GLYCOSYL HYDROLASES
HORMONES
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
METABOLITES
NUCLEI
O-GLYCOSYL HYDROLASES
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HORMONES
RADIOISOTOPES
SEPARATION PROCESSES
SULFUR COMPOUNDS
SYNTHESIS
THYROID HORMONES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques