Molecular and recombinational mapping of mutations in the Ace locus of Drosophila melanogaster

Journal Article · Sun Nov 01 00:00:00 EST 1987 · Genetics; (United States)

OSTI ID:5302474

Nagoshi, R N; Gelbart, W M

The Ace locus in Drosophila melanogaster is known to be the structural gene for acetylcholinesterase. Ace is located in a region of chromosome arm 3R which has been subjected to intensive genetic and molecular analysis. Previous deletion mapping studies have identified a 40-kb region with which the Ace gene resides. This report focuses on the further localization of Ace within this 40-kb interval. Within this region, selective fine structure recombinational analysis was employed to localize three recessive Ace lethals relative to unselected restriction site variations. These three mutations fall into a segment of 7 kb within the Ace interval. Fine structure recombinational analysis was also used to confirm that the Ace/sup -/ phenotype of one deletion, Df(3R)Ace/sup HD1/, co-segregated with the molecular deletion. This deletion does not fully remove Ace activity, but it behaves as a recessive Ace lethal. Df(3R)Ace/sup HD1/ is the most distal Ace lesion identified and indicates that the Ace locus must extend at least 16 kb. Several poly(A)transcripts are detectable in the region defined by the Ace lesions. The position and extent of the Ace locus, as well as the types of transcripts found, is consistent with the recent findings which identified Torpedo-AChE homologous cDNA sequences in this region.

• Research Organization: Harvard Univ. Cambridge, MA
• OSTI ID: 5302474
• Journal Information: Genetics; (United States), Vol. 117:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
DROSOPHILA
GENETIC MAPPING
RECOMBINANT DNA
AUTORADIOGRAPHY
ACETYLCHOLINE
CHOLINESTERASE
ELECTROPHORESIS
GENE MUTATIONS
GENE RECOMBINATION
HYBRIDIZATION
NUCLEOTIDES
PHOSPHORUS 32
AMINES
AMMONIUM COMPOUNDS
ANIMALS
ARTHROPODS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLESTERASES
DAYS LIVING RADIOISOTOPES
DIPTERA
DNA
DRUGS
ENZYMES
ESTERASES
ESTERS
FLIES
FRUIT FLIES
HYDROLASES
INSECTS
INVERTEBRATES
ISOTOPES
LIGHT NUCLEI
MAPPING
MUTATIONS
NEUROREGULATORS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASYMPATHOMIMETICS
PHOSPHORUS ISOTOPES
QUATERNARY COMPOUNDS
RADIOISOTOPES
550401* - Genetics- Tracer Techniques
550601 - Medicine- Unsealed Radionuclides in Diagnostics