Cell cycle-dependent radiosensitivity in two-cell mouse embryos in culture

Journal Article · Fri Feb 01 00:00:00 EST 1980 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575399· OSTI ID:5300345

Domon, M

The radiosensitivity in embryo systems varies depending on factors such as genetic background, oxygen environment, developmental stage, and age of the embryo in cell cycle. This paper is concerned with the involvement of cell cycle age in radiosensitivity of two-cell mouse embryos. Thus the doses needed for 50% killing of blastocyst formation in vitro (LD/sub 50/) of X rays for the two-cell mouse embryos in culture were measured during their cell cycle. The cell cycle in the two-cell embryos was quite peculiar; the cell cycle time of 18 h was divided into a long DNA post synthesis phase (G/sub 2/) plus mitosis (M) of 14 h and a short DNA synthesis phase (S) of 4 h. Results indicate that the LD/sub 50/ varies roughly from 100 to 600 rad within the cell cycle. Thus a major factor in determining the sensitivity to ionizing radiation of two-cell mouse embryos in vitro and perhaps in vivo is their position in the cell division cycle at the time of irradiation.

• Research Organization: Tokyo Medical and Dental Univ., Japan
• OSTI ID: 5300345
• Journal Information: Radiat. Res.; (United States), Vol. 81:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CELL CYCLE
RADIOSENSITIVITY EFFECTS
EMBRYOS
RADIOSENSITIVITY
X RADIATION
BIOLOGICAL RADIATION EFFECTS
AGE DEPENDENCE
CELL CULTURES
DNA
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
GENETICS
ISOLATED VALUES
MICE
MITOSIS
ONTOGENESIS
OXYGEN
TIME DEPENDENCE
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGY
CELL DIVISION
CRYOGENIC FLUIDS
DATA
DATA FORMS
ELECTROMAGNETIC RADIATION
ELEMENTS
FLUIDS
INFORMATION
IONIZING RADIATIONS
MAMMALS
NONMETALS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RODENTS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)