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Title: cDNA isolated from a human T-cell library encodes a member of the protein-tyrosine-phosphatase family

Abstract

A human peripheral T-cell cDNA library was screened with two labeled synthetic oligonucleotides encoding regions of a human placenta protein-tyrosine-phosphatase. One positive clone was isolated and the nucleotide sequence was determined. It contained 1,305 base pairs of open reading frame followed by a TAA stop codon and 978 base pairs of 3{prime} untranslated end, although a poly(A){sup +} tail was not found. An initiator methionine residue was predicted at position 61, which would result in a protein of 415 amino acid residues. This was supported by the synthesis of a M{sub r} 48,000 protein in an in vitro reticulocyte lysate translation system using RNA transcribed from the cloned cDNA and T7 RNA polymerase. The deduced amino acid sequence was compared to other known proteins revealing 65% identity to the low M{sub r} PTPase 1B isolated from placenta. In view of the high degree of similarity, the T-cell cDNA likely encodes a newly discovered protein-tyrosine-phosphatase, thus expanding this family of genes.

Authors:
; ; ; ; ;  [1]
  1. Univ. of Washington, Seattle (USA)
Publication Date:
OSTI Identifier:
5300083
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (USA)
Additional Journal Information:
Journal Volume: 86:14; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PHOSPHATASES; AMINO ACID SEQUENCE; RECOMBINANT DNA; DNA SEQUENCING; ELECTROPHORESIS; LYMPHOCYTES; METHIONINE; MOLECULAR STRUCTURE; OLIGONUCLEOTIDES; PHOSPHORUS 32; PHOSPHORYLATION; PLACENTA; SULFUR 35; TYROSINE; AMINO ACIDS; ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DNA; DRUGS; ENZYMES; ESTERASES; EVEN-ODD NUCLEI; FETAL MEMBRANES; HYDROLASES; HYDROXY ACIDS; ISOTOPES; LEUKOCYTES; LIGHT NUCLEI; LIPOTROPIC FACTORS; MATERIALS; MEMBRANES; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PHOSPHORUS ISOTOPES; RADIOISOTOPES; SOMATIC CELLS; STRUCTURAL CHEMICAL ANALYSIS; SULFUR ISOTOPES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Cool, D E, Tonks, N K, Charbonneau, H, Walsh, K A, Fischer, E H, and Krebs, E G. cDNA isolated from a human T-cell library encodes a member of the protein-tyrosine-phosphatase family. United States: N. p., 1989. Web. doi:10.1073/pnas.86.14.5257.
Cool, D E, Tonks, N K, Charbonneau, H, Walsh, K A, Fischer, E H, & Krebs, E G. cDNA isolated from a human T-cell library encodes a member of the protein-tyrosine-phosphatase family. United States. https://doi.org/10.1073/pnas.86.14.5257
Cool, D E, Tonks, N K, Charbonneau, H, Walsh, K A, Fischer, E H, and Krebs, E G. 1989. "cDNA isolated from a human T-cell library encodes a member of the protein-tyrosine-phosphatase family". United States. https://doi.org/10.1073/pnas.86.14.5257.
@article{osti_5300083,
title = {cDNA isolated from a human T-cell library encodes a member of the protein-tyrosine-phosphatase family},
author = {Cool, D E and Tonks, N K and Charbonneau, H and Walsh, K A and Fischer, E H and Krebs, E G},
abstractNote = {A human peripheral T-cell cDNA library was screened with two labeled synthetic oligonucleotides encoding regions of a human placenta protein-tyrosine-phosphatase. One positive clone was isolated and the nucleotide sequence was determined. It contained 1,305 base pairs of open reading frame followed by a TAA stop codon and 978 base pairs of 3{prime} untranslated end, although a poly(A){sup +} tail was not found. An initiator methionine residue was predicted at position 61, which would result in a protein of 415 amino acid residues. This was supported by the synthesis of a M{sub r} 48,000 protein in an in vitro reticulocyte lysate translation system using RNA transcribed from the cloned cDNA and T7 RNA polymerase. The deduced amino acid sequence was compared to other known proteins revealing 65% identity to the low M{sub r} PTPase 1B isolated from placenta. In view of the high degree of similarity, the T-cell cDNA likely encodes a newly discovered protein-tyrosine-phosphatase, thus expanding this family of genes.},
doi = {10.1073/pnas.86.14.5257},
url = {https://www.osti.gov/biblio/5300083}, journal = {Proceedings of the National Academy of Sciences of the United States of America; (USA)},
issn = {0027-8424},
number = ,
volume = 86:14,
place = {United States},
year = {Sat Jul 01 00:00:00 EDT 1989},
month = {Sat Jul 01 00:00:00 EDT 1989}
}