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Title: Primary structure of bovine pituitary secretory protein I (chromogranin A) deduced from the cDNA sequence

Abstract

Secretory protein I (SP-I), also referred to as chromogranin A, is an acidic glycoprotein that has been found in every tissue of endocrine and neuroendocrine origin examined but never in exocrine or epithelial cells. Its co-storage and co-secretion with peptide hormones and neurotransmitters suggest that it has an important endocrine or secretory function. The authors have isolated cDNA clones from a bovine pituitary lambdagt11 expression library using an antiserum to parathyroid SP-I. The largest clone (SP4B) hybridized to a transcript of 2.1 kilobases in RNA from parathyroid, pituitary, and adrenal medulla. Immunoblots of bacterial lysates derived from SP4B lysognes demonstrated specific antibody binding to an SP4B/..beta..-galactosidase fusion protein (160 kDa) with a cDNA-derived component of 46 kDa. Radioimmunoassay of the bacterial lystates with SP-I antiserum yielded parallel displacement curves of /sup 125/I-labeled SP-I by the SP4B lysate and authentic SP-I. SP4B contains a cDNA of 1614 nucleotides that encodes a 449-amino acid protein (calculated mass, 50 kDa). The nucleotide sequences of the pituitary SP-I cDNA and adrenal medullary SP-I cDNAs are nearly identical. Analysis of genomic DNA suggests that pituitary, adrenal, and parathyroid SP-I are products of the same gene.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Johns Hopkins Univ. School of Medicine, Baltimore, MD (USA)
OSTI Identifier:
5293961
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 84:14
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; GLUCOPROTEINS; MOLECULAR STRUCTURE; RADIOIMMUNOASSAY; PITUITARY GLAND; BIOCHEMISTRY; RECOMBINANT DNA; DNA SEQUENCING; ADRENAL GLANDS; CATTLE; IODINE 125; PARATHYROID GLANDS; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; CARBOHYDRATES; CHEMISTRY; DAYS LIVING RADIOISOTOPES; DNA; DOMESTIC ANIMALS; ELECTRON CAPTURE RADIOISOTOPES; ENDOCRINE GLANDS; GLANDS; IMMUNOASSAY; IMMUNOLOGY; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; NUCLEI; NUCLEIC ACIDS; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOASSAY; RADIOIMMUNOLOGY; RADIOISOTOPES; RUMINANTS; SACCHARIDES; STRUCTURAL CHEMICAL ANALYSIS; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Ahn, T G, Cohn, D V, Gorr, S U, Ornstein, D L, Kashdan, M A, and Levine, M A. Primary structure of bovine pituitary secretory protein I (chromogranin A) deduced from the cDNA sequence. United States: N. p., 1987. Web. doi:10.1073/pnas.84.14.5043.
Ahn, T G, Cohn, D V, Gorr, S U, Ornstein, D L, Kashdan, M A, & Levine, M A. Primary structure of bovine pituitary secretory protein I (chromogranin A) deduced from the cDNA sequence. United States. https://doi.org/10.1073/pnas.84.14.5043
Ahn, T G, Cohn, D V, Gorr, S U, Ornstein, D L, Kashdan, M A, and Levine, M A. 1987. "Primary structure of bovine pituitary secretory protein I (chromogranin A) deduced from the cDNA sequence". United States. https://doi.org/10.1073/pnas.84.14.5043.
@article{osti_5293961,
title = {Primary structure of bovine pituitary secretory protein I (chromogranin A) deduced from the cDNA sequence},
author = {Ahn, T G and Cohn, D V and Gorr, S U and Ornstein, D L and Kashdan, M A and Levine, M A},
abstractNote = {Secretory protein I (SP-I), also referred to as chromogranin A, is an acidic glycoprotein that has been found in every tissue of endocrine and neuroendocrine origin examined but never in exocrine or epithelial cells. Its co-storage and co-secretion with peptide hormones and neurotransmitters suggest that it has an important endocrine or secretory function. The authors have isolated cDNA clones from a bovine pituitary lambdagt11 expression library using an antiserum to parathyroid SP-I. The largest clone (SP4B) hybridized to a transcript of 2.1 kilobases in RNA from parathyroid, pituitary, and adrenal medulla. Immunoblots of bacterial lysates derived from SP4B lysognes demonstrated specific antibody binding to an SP4B/..beta..-galactosidase fusion protein (160 kDa) with a cDNA-derived component of 46 kDa. Radioimmunoassay of the bacterial lystates with SP-I antiserum yielded parallel displacement curves of /sup 125/I-labeled SP-I by the SP4B lysate and authentic SP-I. SP4B contains a cDNA of 1614 nucleotides that encodes a 449-amino acid protein (calculated mass, 50 kDa). The nucleotide sequences of the pituitary SP-I cDNA and adrenal medullary SP-I cDNAs are nearly identical. Analysis of genomic DNA suggests that pituitary, adrenal, and parathyroid SP-I are products of the same gene.},
doi = {10.1073/pnas.84.14.5043},
url = {https://www.osti.gov/biblio/5293961}, journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 84:14,
place = {United States},
year = {Wed Jul 01 00:00:00 EDT 1987},
month = {Wed Jul 01 00:00:00 EDT 1987}
}