Differential immune response of congenic mice to ultraviolet-treated major histocompatibility complex class II-incompatible skin grafts

Journal Article · Tue Mar 01 00:00:00 EST 1988 · Transplantation; (United States)

DOI:https://doi.org/10.1097/00007890-198803000-00020· OSTI ID:5288893

Vermeer, B J; Santerse, B; Van De Kerckhove, B A; Schothorst, A A; Claas, F H

The influence of ultraviolet (UVB) irradiation on the survival of H-2 class II-disparate skin grafts was studied in congenic mouse strains. Isolated skin was UVB irradiated in vitro at a dose of 40 mJ/cm/sup 2/ from both sides to remove Ia immunogenicity. Immediately after irradiation the skin was transplanted onto the flank of allogeneic mice. When B10.AQR grafts were transplanted onto B10.T(6R) recipients, a significant prolongation of the survival time was observed, while 50% of the UVB-treated grafts were not rejected at all. However, in the opposite direction--i.e., B10.T(6R) grafts onto B10.AQR recipients, no significant prolongation of the survival was observed. To test whether this effect was due to a difference in susceptibility of the donor skin to UVB irradiation or to a different immune response in the recipients, (B10.T(6R) x B10.AQR) grafts were transplanted onto the parent strains. Similar results were obtained, in that UVB-treated grafts did not show a prolonged survival in B10.AQR recipients, whereas a significant prolongation (50% of the grafts survived more than 100 days) was observed in B10.T(6R) recipients. UVB-treated (B10.T(6R) x B10.AQR)F1 grafts were also transplanted onto (B10.T(6R) x C57B1/10)F1, (B10.AQR x C57B1/10)F1, (B10.T(6R) x Balb/c)F1 and (B10.AQR x Balb/c)F1 recipients--but in none of these combinations was a prolonged survival time observed. These data suggest that, in contrast to all in vitro experiments, the abrogation of the immune response by UVB treatment of the stimulator cells is, in vivo, not a general phenomenon. The genetic constitution of the responder mice seems to play an important role in determining whether or not an immune response takes place.

• Research Organization: Univ. Medical Center, Leiden (Netherlands)
• OSTI ID: 5288893
• Journal Information: Transplantation; (United States), Vol. 45:3
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
IMMUNE REACTIONS
RADIOINDUCTION
SKIN
BIOLOGICAL RADIATION EFFECTS
TRANSPLANTS
ANTIBODY FORMATION
IN VITRO
MICE
SURVIVAL TIME
ULTRAVIOLET RADIATION
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MAMMALS
ORGANS
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RADIATIONS
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560120* - Radiation Effects on Biochemicals
Cells
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