Thermal stability of Trichoderma reesei C30 cellulase and Aspergillus niger. beta. -glucosidase after pH and chemical modification

Conference · Thu Jan 01 00:00:00 EST 1981 · Biotechnol. Bioeng. Symp.; (United States)

OSTI ID:5287348

Woodward, J; Whaley, K S; Zachry, G S; Wohlpart, D L

Treatment of Trichoderma reesei C30 cellulase at pH 10.0 for 1 h at room temperature increased its pH and thermal stability. Chemical modification of the free epsilon-amino groups of cellulase at pH 10.0 resulted in no further increase in stability. Such chemical modification, however, decreased the thermal stability of the cellulose-cellulase complex. On the contrary, the chemical modification of Aspergillus niger ..beta..-glucosidase with glutaraldehyde at pH 8.0 increased the thermal stability of this enzyme.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 5287348
• Report Number(s): CONF-810554-
• Journal Information: Biotechnol. Bioeng. Symp.; (United States), Vol. 11; Conference: 3. symposium on biotechnology in energy production and conservation, Gatlinburg, TN, USA, 12 May 1981
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
CELLULASE
STABILITY
GLUCOSIDASE
CELLULOSE
HYDROLYSIS
MODIFICATIONS
PH VALUE
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
LYSIS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
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