De novo synthesis of purine nucleotides in different fiber types of rat skeletal muscle

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5278674

Tullson, P C; John-Alder, H; Hood, D A; Terjung, R L

The contribution of de novo purine nucleotide synthesis to nucleotide metabolism in skeletal muscles is not known. The authors have determined rates of de novo synthesis in soleus (slow-twitch red), red gastrocnemius (fast-twitch red), and white gastrocnemius (fast-twitch white) using the perfused rat hindquarter. /sup 14/C glycine incorporation into ATP was linear after 1 and 2 hours of perfusion with 0.2 mM added glycine. The intracellular (I) and extracellular (E) specific activity of /sup 14/C glycine was determined by HPLC of phenylisothiocyanate derivatives of neutralized PCA extracts. The rates of de novo synthesis when expressed relative to muscle ATP content show slow and fast-twitch red muscles to be similar and about twice as great as fast-twitch white muscles. This could represent a greater turnover of the adenine nucleotide pool in more oxidative red muscle types.

• Research Organization: State Univ. of New York, Syracuse
• OSTI ID: 5278674
• Report Number(s): CONF-8604222-; TRN: 86-030414
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MUSCLES
BIOCHEMISTRY
NUCLEOTIDES
BIOSYNTHESIS
ATP
CARBON 14 COMPOUNDS
FIBERS
GLYCINE
LIQUID COLUMN CHROMATOGRAPHY
PERFUSED TISSUES
RATS
TRACER TECHNIQUES
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
BODY
CARBOXYLIC ACIDS
CHEMISTRY
CHROMATOGRAPHY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
RODENTS
SEPARATION PROCESSES
SYNTHESIS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques