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Title: Acylation and metabolism of (n-6) fatty acids in hepatocytes

Isolated hepatocytes (5 x 10/sup 6/ in 2ml) from chow fed rats were incubated from 20 to 60 min. with increasing concentrations of (1-/sup 14/C) labeled 18:2 (n-6), 18:3 (n-6) or 20:3 (n-6) to define optimum conditions for measuring acylation and metabolism to other (n-6) acids with subsequent incorporation into lipids. The triglycerides (TG) and phospholipids (PL) contained 157 and 80 nmols of 18:2 (n-6) and 6.0 and 6.1 nmols of other (n-6) acids, respectively, when cells were incubated with 0.3mM (1-/sup 14/C) 18:2 (n-6) for 40 min. When cells were incubated with 0.3mM (1-/sup 14/C) 18:2 (n-6) plus 0.15 to 0.45mM 18:3 (n-6) or 20:3 (n-6), the metabolism of 18:2 (n-6) to other (n-6) acids was inhibited but not totally abolished. These results may suggest that (n-6) acid made from linoleate do not totally equilibrate with exogenous 18:3 (n-6) or 20:3 (n-6).
Authors:
;
Publication Date:
OSTI Identifier:
5277863
Report Number(s):
CONF-8606151-
Journal ID: CODEN: FEPRA
Resource Type:
Conference
Resource Relation:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States); Journal Volume: 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
Research Org:
Ohio State Univ., Columbus
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CARBOXYLIC ACIDS; ACYLATION; METABOLISM; CARBON 14 COMPOUNDS; LIVER CELLS; PHOSPHOLIPIDS; RATS; TRACER TECHNIQUES; TRIGLYCERIDES; ANIMAL CELLS; ANIMALS; CHEMICAL REACTIONS; ESTERS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LIPIDS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; RODENTS; SOMATIC CELLS; VERTEBRATES 550501* -- Metabolism-- Tracer Techniques