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Title: Effect of oral administration of mutagens found in food on the frequency of sister chromatid exchanges in the colonic epithelium of mice

Abstract

Epidemiological studies indicate there is a link between dietary factors and the incidence of colon cancer, and it has been suggested mutagens in foods might be responsible for initiating the carcinogenic process. Some food mutagens are formed during the cooking process. For example, certain heterocyclic amines, including Trp-P-2 (3-amino-1-methyl-5H-pyrido(4,3-n) indole) and MeIQ (2-amino-3,4-dimethylimidazo(4,5-f)quinoline), which have been isolated from broiled meat and fish at low (ng/g) levels, are extremely potent mutagens in the Ames Salmonella/microsome test and can induce mutation in cultured mammalian cells as well. Other mutagens in foods are natural products; quercetin, a flavanoid widely distributed in plant products, is mutagenic to Salmonella and cultured mammalian cells. As most of the evidence implicating substance in food as mutagenic carcinogens comes from in vitro studies, it is of interest to determine whether these compounds can also exert genotoxic effects in vivo, particularly in colonic tissue. The ability to induce nuclear aberrations in vivo in murine colonic epithelial tissue has been suggested to be a property of colon carcinogens specifically, and several mutagens found in cooked food, including MeIQ and Trp-P-2, have been found to produce such nucleotoxicity. The authors report here tests of the ability of MeIQ, Trp-P-2, and quercetinmore » to induce sister chromatid exchanges (SCEs) in the colonic epithelium of mice.« less

Authors:
; ;
Publication Date:
Research Org.:
Ludwig Institute for Cancer Research, Toronto, Ontario (Canada)
OSTI Identifier:
5276942
Resource Type:
Journal Article
Journal Name:
Environ. Mutagen.; (United States)
Additional Journal Information:
Journal Volume: 10:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; INDOLES; GENETIC EFFECTS; MUTAGENS; ORAL ADMINISTRATION; QUERCETIN; QUINOLINES; EPITHELIUM; FOOD; FOOD PROCESSING; IN VIVO; LARGE INTESTINE; MICE; SISTER CHROMATID EXCHANGES; ANIMAL TISSUES; ANIMALS; AROMATICS; AZAARENES; AZINES; AZOLES; BIOLOGICAL EFFECTS; BODY; CHROMOSOMAL ABERRATIONS; DIGESTIVE SYSTEM; FLAVENOIDS; FLAVONES; GASTROINTESTINAL TRACT; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INTESTINES; MAMMALS; MUTATIONS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; ORGANS; PHENOLS; POLYPHENOLS; PROCESSING; PYRANS; PYRIDINES; PYRROLES; RODENTS; TISSUES; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Couch, D B, Stuart, E, and Heddle, J A. Effect of oral administration of mutagens found in food on the frequency of sister chromatid exchanges in the colonic epithelium of mice. United States: N. p., 1987. Web. doi:10.1002/em.2850100211.
Couch, D B, Stuart, E, & Heddle, J A. Effect of oral administration of mutagens found in food on the frequency of sister chromatid exchanges in the colonic epithelium of mice. United States. https://doi.org/10.1002/em.2850100211
Couch, D B, Stuart, E, and Heddle, J A. 1987. "Effect of oral administration of mutagens found in food on the frequency of sister chromatid exchanges in the colonic epithelium of mice". United States. https://doi.org/10.1002/em.2850100211.
@article{osti_5276942,
title = {Effect of oral administration of mutagens found in food on the frequency of sister chromatid exchanges in the colonic epithelium of mice},
author = {Couch, D B and Stuart, E and Heddle, J A},
abstractNote = {Epidemiological studies indicate there is a link between dietary factors and the incidence of colon cancer, and it has been suggested mutagens in foods might be responsible for initiating the carcinogenic process. Some food mutagens are formed during the cooking process. For example, certain heterocyclic amines, including Trp-P-2 (3-amino-1-methyl-5H-pyrido(4,3-n) indole) and MeIQ (2-amino-3,4-dimethylimidazo(4,5-f)quinoline), which have been isolated from broiled meat and fish at low (ng/g) levels, are extremely potent mutagens in the Ames Salmonella/microsome test and can induce mutation in cultured mammalian cells as well. Other mutagens in foods are natural products; quercetin, a flavanoid widely distributed in plant products, is mutagenic to Salmonella and cultured mammalian cells. As most of the evidence implicating substance in food as mutagenic carcinogens comes from in vitro studies, it is of interest to determine whether these compounds can also exert genotoxic effects in vivo, particularly in colonic tissue. The ability to induce nuclear aberrations in vivo in murine colonic epithelial tissue has been suggested to be a property of colon carcinogens specifically, and several mutagens found in cooked food, including MeIQ and Trp-P-2, have been found to produce such nucleotoxicity. The authors report here tests of the ability of MeIQ, Trp-P-2, and quercetin to induce sister chromatid exchanges (SCEs) in the colonic epithelium of mice.},
doi = {10.1002/em.2850100211},
url = {https://www.osti.gov/biblio/5276942}, journal = {Environ. Mutagen.; (United States)},
number = ,
volume = 10:2,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1987},
month = {Thu Jan 01 00:00:00 EST 1987}
}