An investigation of liquid secondary ion and laser desorption mass spectroscopy for the analysis of planar chromatograms

Technical Report · Thu Nov 01 00:00:00 EST 1990

OSTI ID:5275370

Dunphy, J C

In the work described in this dissertation, interfaces between two mass spectrometric methods, liquid secondary ion mass spectrometry (LSIMS) and laser desorption/ionization Fourier transform mass spectrometry (LD/FTMS), and thin-layer chromatography (TLC) and slab gel electrophoresis were developed for bioanalytical applications. In an investigation of direct LSIMS for TLC analysis (TLC/LSIMS), mass spectra of bile acids and bile salts were characterized directly from high-performance TLC plates. The scanning ability of the LSIMS instrument was used to generate spatial profiles of the characteristic bile acid ions in the mass spectra. A procedure for the analysis of bile salts in dog bile was developed involving an extraction step, followed by TLC separation and direct TLC/LSIMS detection and semi-quantitation. For peptides, an experiment called selected-sequence monitoring'' was developed to locate target peptides related in structure in complex mixtures developed on TLC plates. Ions characteristic of the bradykinin and enkephalin peptides were used to generate spatial profiles of members of those peptide families on TLC plates. Using a Fourier transform mass spectrometer (FTMS), a fundamental investigation was conducted into the factors affecting the quality of analytical data obtained using direct laser desorption/ionization to produce mass spectra from TLC plates.

• Research Organization: Indiana Univ., Bloomington, IN (United States). Dept. of Chemistry
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: AC05-76OR00033
• OSTI ID: 5275370
• Report Number(s): DOE/OR/00033-T475; ON: DE92013655
• Resource Relation: Other Information: Thesis (Ph.D.)
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MATERIALS
CHEMICAL ANALYSIS
SEPARATION PROCESSES
BILE ACIDS
BRADYKININ
DESORPTION
ELECTROPHORESIS
ENKEPHALINS
FOURIER TRANSFORM SPECTROMETERS
GELS
MASS SPECTROSCOPY
OLIGONUCLEOTIDES
POLYAMIDES
THIN-LAYER CHROMATOGRAPHY
AUTONOMIC NERVOUS SYSTEM AGENTS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
COLLOIDS
DISPERSIONS
DRUGS
ENDORPHINS
HYDROXY COMPOUNDS
KININS
MATERIALS
MEASURING INSTRUMENTS
NEUROREGULATORS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PEPTIDES
POLYMERS
POLYPEPTIDES
PROTEINS
SPECTROMETERS
SPECTROSCOPY
STEROIDS
STEROLS
400102* - Chemical & Spectral Procedures
400105 - Separation Procedures
550200 - Biochemistry