/sup 31/P NMR analysis of membrane phospholipid organization in viable, reversibly electropermeabilized Chinese hamster ovary cells
Chinese hamster ovary (CHO) cells were reversibly permeabilized by submitting them to short, high-intensity, square wave pulses (1.8 kV/cm, 100 ..mu..s). The cells remained in a permeable state without loss of viability for several hours at 4/sup 0/C. A new anisotropic peak with respect to control cells was observed on /sup 31/P NMR spectroscopic analysis of the phospholipid components. This peak is only present when the cells are permeable, and normal anisotropy is recovered after resealing. Taking into account the fusogenicity of electropermeabilized cells, comparative studies were performed on 5% poly(ethylene glycol) treated cells. The /sup 31/P NMR spectra of the phospholipids displayed the same anisotropic peak as in the case of the electropermeabilized cells. In the two cases, this anisotropic peak was located downfield from the main peak associated to the phospholipids when organized in bilayers. The localization of this anisotropic peak is very different from the one of a hexagonal phase. The authors proposed a reorganization of the polar head group region leading to a weakening of the hydration layer to account for these observations. This was also thought to explain the electric field induced fusogenicity of these cells.
- Research Organization:
- Centre de Recherches de Biochimie et de Genetique Cellulaires du CNRS, Toulouse (France)
- OSTI ID:
- 5271457
- Journal Information:
- Biochemistry; (United States), Vol. 27:4
- Country of Publication:
- United States
- Language:
- English
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CELL MEMBRANES
NUCLEAR MAGNETIC RESONANCE
PERMEABILITY
PHOSPHOLIPIDS
BIOCHEMISTRY
ANISOTROPY
CHO CELLS
PHOSPHORUS 31
ANIMAL CELLS
CELL CONSTITUENTS
CHEMISTRY
ESTERS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAGNETIC RESONANCE
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
RESONANCE
STABLE ISOTOPES
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