Multiorgan crystal deposition following intravenous oxalate infusion in rat

Journal Article · Sun Jun 01 00:00:00 EDT 1986 · J. Urol.; (United States)

OSTI ID:5267772

Blumenfrucht, M J; Cheeks, C; Wedeen, R P

Deposition of calcium oxalate is responsible for the pathologic manifestations of oxalosis and may contribute to multiorgan dysfunction in uremia and to the progression of renal damage after renal failure is established. We have developed a rat model of oxalosis using a single intravenous injection of sodium oxalate, 0.3 mmol./kg. body weight, in rats. Polarized light microscopy and section freeze-dry autoradiography were used to identify /sup 14/C-oxalate within the renal parenchyma and in extrarenal organs. /sup 14/C-oxalate crystals under three mu in length were identified within one min. of injection in proximal tubule lumens. Section freeze-dry autoradiography showed occasional minute crystals within glomeruli, heart, lung and liver at one hr. In contrast to concentrative cellular uptake demonstrated in rat renal cortical slices in vitro, intracellular accumulation of /sup 14/C-oxalate could not be detected in vivo. Within the first 24 hr., renal oxalate retention reached a maximum of 25 +/- 4 per cent of the injected dose/gm. kidney compared to a maximum of only 7 +/- 3 per cent/gm. kidney after intraperitoneal administration. Although less than one per cent dose/gm. kidney remained after one week, crystal fragments were scattered throughout the cortex and medulla, often surrounded by foci of interstitial nephritis. The retention of crystals in kidney and other body organs following i.v. oxalate provides a model of oxalosis which stimulates pathophysiologic events in a variety of clinical situations characterized by transiently or persistently elevated serum oxalate.

• Research Organization: Veterans Administration Medical Center, East Orange, NJ
• OSTI ID: 5267772
• Journal Information: J. Urol.; (United States), Vol. 6
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
OXALATES
CRYSTALLIZATION
TISSUE DISTRIBUTION
AUTORADIOGRAPHY
HEART
KIDNEYS
LIVER
LUNGS
METABOLIC DISEASES
RATS
ANIMALS
BODY
CARBOXYLIC ACID SALTS
CARDIOVASCULAR SYSTEM
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
GLANDS
MAMMALS
ORGANS
PHASE TRANSFORMATIONS
RESPIRATORY SYSTEM
RODENTS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550501 - Metabolism- Tracer Techniques