Continuous transformation of benzaldehyde to benzyl alcohol by Rhodotorula mucilaginosa immobilized in an ultrafiltration cell

Journal Article · Tue Jun 01 00:00:00 EDT 1982 · Biotechnol. Bioeng.; (United States)

OSTI ID:5265692

Wisniewski, J; Winnicki, T; Majewska, K

Microbiological transformation of benzaldehyde accomplished by the fungus Rhodotorula mucilaginosa immobilized in the ultrafiltration cell was studied. A polysulfone membrane formed on a sintered PVC support was used for the separation of the transformation product from the cellular material. Kinetic investigations have led to results which are typical of continuously fed stirred tank reactors (CFSTR)-the value of the maximum reaction rate (Vmax) and apparent Michaelis constant (K'm) are practically independent of the substrate retention time (calculated in terms of the flow intensity value). A strong relationship was found to occur between Vmax and biomass concentration in the reactor. Study of the apparent enzyme stability shows that the decrease in the biocatalyst activity is chiefly caused by penetration of the cells through the membrane. The experimental results were approximated in terms of the adopted mathematical model. Based on this model, the half-lives (t1/2) of enzyme activities were determined. The t1/2 value varies from 35 to 82 days and depends both on the permeate flux through the membrane and on the separation properties of the membrane. (Refs. 15).

• Research Organization: Inst. of Environmental Protection Engineering, Technical Univ. of Wroclaw, Pl Grunwaldzki 9, Wroclaw, Poland
• OSTI ID: 5265692
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 25:6
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
BENZALDEHYDE
BIOCONVERSION
FUNGI
ENZYME ACTIVITY
IMMOBILIZED CELLS
BENZYL ALCOHOL
BIOCHEMICAL REACTION KINETICS
LIQUID WASTES
MATHEMATICAL MODELS
MEMBRANES
ULTRAFILTRATION
ALCOHOLS
ALDEHYDES
AROMATICS
FILTRATION
HYDROXY COMPOUNDS
KINETICS
ORGANIC COMPOUNDS
PLANTS
REACTION KINETICS
SEPARATION PROCESSES
WASTES
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
550700 - Microbiology