Regulation of collagen synthesis in human dermal fibroblasts by ascorbic-induced lipid peroxidation

Conference · Mon Mar 11 00:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5264080

Geesin, J C ^[1]; Gordon, J S ^[2]; Gordon, J S ^[2]; Berg, R A ^[3]

---

1. Robert Wood Johnson Medical School, Piscataway, NJ (United States) Johnson and Johnson Consumer Products, Inc., Skillman, NJ (United States)
2. Johnson and Johnson Consumer Products, Inc., Skillman, NJ (United States)
3. Robert Wood Johnson Medical School, Piscataway, NJ (United States)

Ascorbic acid has been shown to stimulate collagen synthesis through the induction of lipid peroxidation which leads to increased transcription of the collagen genes. To test the ability of aldehyde products of lipid peroxidation to mediate this effect, the authors treated cultured fibroblasts with 1-200{mu}M of malondialdehyde, acetaldehyde, glyoxal or hexenal in the presence of lipid peroxidation inducing or noninducing concentrations of ascorbic acid. The treatment process involved either pretreatment of cells for 66hrs with either concentration of ascorbate before a 6hr treatment in the presence of ascorbate and the aldehydes, or 6 or 72hr treatment of the cells in the presence of either concentration of ascorbate plus the aldehydes. No effect of any of these aldehydes was seen on ascorbate-stimulated collagen synthesis. Also, pretreatment of fibroblasts for 24hrs with 100nM phorbol myristate acetate (PMA), which produces down regulation of protein kinase C(PKC), failed to alter the ascorbate-stimulation of collagen synthesis. Additionally, the authors tested the ability of benzamide, a poly ACP ribosylation inhibitor, to inhibit the ascorbate response with no specific effect noted. These results do not support the proposed roles for aldehydes, PKC, or poly ADP ribosylation in the mediation of the lipid peroxidation induced stimulation of collagen synthesis.

• OSTI ID: 5264080
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
COLLAGEN
BIOSYNTHESIS
LIPIDS
METABOLISM
PHORBOL ESTERS
BIOLOGICAL EFFECTS
AMIDES
ASCORBIC ACID
FIBROBLASTS
GENES
INHIBITION
MAN
PHOSPHOTRANSFERASES
TRANSCRIPTION
ANIMAL CELLS
ANIMALS
CARCINOGENS
CONNECTIVE TISSUE CELLS
ENZYMES
ESTERS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PRIMATES
PROTEINS
SCLEROPROTEINS
SOMATIC CELLS
SYNTHESIS
TRANSFERASES
VERTEBRATES
VITAMINS
560300* - Chemicals Metabolism & Toxicology