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Title: Mitotic recombination of chromosome 17 in astrocytomas

Allelic combinations at seven loci on human chromosome 17 defined by restriction fragment length polymorphisms were determined in tumor and normal tissues from 35 patients with gliomas. Loss of constitutional heterozygosity at one or more of these loci was observed in 8 of the 24 tumors displaying astrocytic differentiation and in the single primitive neuroectodermal tumor examined. The astrocytomas showing these losses included examples of each adult malignancy grade of the disease, including glioblastoma (malignancy grade IV), and seven of them demonstrated concurrent maintenance of heterozygosity for at least one chromosome 17 locus. Determination of allele dosage together with the genotypic data indicated that the tumor chromosomes 17 were derived by mitotic recombination in 7 of the 9 cases with shared homozygosity of the region 17p11.2-ptr in all cases. In contrast, tumors of oligodendrocytic, ependymal, or mixed cellular differentiation did not exhibit loss of alleles at any of the loci examined. These data suggest that the somatic attainment of homozygosity for loci on chromosome 17p is frequently associated with the oncogenesis of central nervous system tumors, particularly those showing solely astrocytic differentiation, and that mitotic recombination mapping is a useful approach towards the subregional localization of a locus whose rearrangementmore » is involved in this disease.« less
Authors:
; ; ; ;  [1]
  1. (Ludwig Institute for Cancer Research, Montreal (Canada))
Publication Date:
OSTI Identifier:
5263170
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA); Journal Volume: 86:8
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ASTROCYTOMAS; GENETICS; DNA; AUTORADIOGRAPHY; HETEROCHROMOSOMES; GENE RECOMBINATION; CHROMOSOMAL ABERRATIONS; MAN; RFLPS; ANIMALS; BIOLOGY; CHROMOSOMES; DISEASES; GLIOMAS; MAMMALS; MUTATIONS; NEOPLASMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PRIMATES; VERTEBRATES 550401* -- Genetics-- Tracer Techniques