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Title: Human cDNA clones for an. cap alpha. subunit of G/sub i/ signal-transduction protein

Two cDNA clones were obtained from a lambdagt11 cDNA human brain library that correspond to ..cap alpha../sub i/ subunits of G signal-transduction proteins (where ..cap alpha../sub i/ subunits refer to the ..cap alpha.. subunits of G proteins that inhibit adenylate cyclase). The nucleotide sequence of human brain ..cap alpha../sub i/ is highly homologous to that of bovine brain ..cap alpha../sub i/ and the predicted amino acid sequences are identical. However, human and bovine brain ..cap alpha../sub i/ cDNAs differ significantly from ..cap alpha../sub i/ cDNAs from human monocytes, rat glioma, and mouse macrophages in amino acid (88% homology) and nucleotide (71-75% homology) sequences. In addition, the nucleotide sequences of the 3' untranslated regions of human and bovine brain ..cap alpha../sub i/ cDNAs differ markedly from the sequences of human monocyte, rat glioma, and mouse macrophage ..cap alpha../sub i/ cDNAs. These results suggest there are at least two classes of ..cap alpha../sub i/ mRNA
Authors:
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Publication Date:
OSTI Identifier:
5242991
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States); Journal Volume: 84:15
Research Org:
National Institutes of Health, Bethesda, MD (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; MESSENGER-RNA; AUTORADIOGRAPHY; PROTEINS; AMINO ACID SEQUENCE; DNA-CLONING; BRAIN; CATTLE; CYCLASES; PHOSPHORUS 32; RECEPTORS; RECOMBINANT DNA; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CENTRAL NERVOUS SYSTEM; CLONING; DAYS LIVING RADIOISOTOPES; DNA; DOMESTIC ANIMALS; ENZYMES; ISOTOPES; LIGHT NUCLEI; LYASES; MAMMALS; MEMBRANE PROTEINS; MOLECULAR STRUCTURE; NERVOUS SYSTEM; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; RADIOISOTOPES; RNA; RUMINANTS; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques