Model for conductometric detection of carbohydrates and alcohols as complexes with boric acid and borate ion in high-performance liquid chromatography

Journal Article · Wed Mar 15 00:00:00 EST 1989 · Analytical Chemistry; (USA)

DOI:https://doi.org/10.1021/ac00181a028· OSTI ID:5242887

Bertrand, G L; Armstrong, D W ^[1]

---

1. Univ. of Missouri, Rolla (USA)

In recent articles, Okada has demonstrated the utility of indirect conductometric detection of electrically neutral sugars and alcohols through their complexes in boric acid solution. The use of a boric acid eluent provides a highly sensitive means of detection for monosaccharides, lactose, and sugar alcohols but not for polysaccharides (other than lactose) and simple alcohols. Addition of sorbitol, mannitol, or fructose to the boric acid eluent allows detection of the polysaccharides and simple alcohols, as well as lactose, glucose, fructose, and presumably other monosaccharides and sugar alcohols. These results were interpreted in terms of the ability of an analyte to form either dissociated or undissociated complexes with boric acid. This interpretation was quantified with a mathematical description of the complexation equilibria and the conductivity due to ionic species. Unfortunately, the mathematical model contains some incorrect assumptions that severely limit the utility of the derived equations and may prevent optimization of this potentially important technique. We present here a more general mathematical model that does not suffer from these limitations.

• DOE Contract Number: FG02-88ER13819
• OSTI ID: 5242887
• Journal Information: Analytical Chemistry; (USA), Vol. 61:6; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
ALCOHOLS
LIQUID COLUMN CHROMATOGRAPHY
BORATES
BORIC ACID
CARBOHYDRATES
MATHEMATICAL MODELS
POTENTIOMETRY
QUALITATIVE CHEMICAL ANALYSIS
THEORETICAL DATA
BORON COMPOUNDS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
INORGANIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
SEPARATION PROCESSES
TITRATION
400102* - Chemical & Spectral Procedures