skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Complete cDNA and derived amino acid sequence of human factor V

Abstract

cDNA clones encoding human factor V have been isolated from an oligo(dT)-primed human fetal liver cDNA library prepared with vector Charon 21A. The cDNA sequence of factor V from three overlapping clones includes a 6672-base-pair (bp) coding region, a 90-bp 5' untranslated region, and a 163-bp 3' untranslated region within which is a poly(A)tail. The deduced amino acid sequence consists of 2224 amino acids inclusive of a 28-amino acid leader peptide. Direct comparison with human factor VIII reveals considerable homology between proteins in amino acid sequence and domain structure: a triplicated A domain and duplicated C domain show approx. 40% identity with the corresponding domains in factor VIII. As in factor VIII, the A domains of factor V share approx. 40% amino acid-sequence homology with the three highly conserved domains in ceruloplasmin. The B domain of factor V contains 35 tandem and approx. 9 additional semiconserved repeats of nine amino acids of the form Asp-Leu-Ser-Gln-Thr-Thr/Asn-Leu-Ser-Pro and 2 additional semiconserved repeats of 17 amino acids. Factor V contains 37 potential N-linked glycosylation sites, 25 of which are in the B domain, and a total of 19 cysteine residues.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Univ. of Vermont, Burlington (USA)
OSTI Identifier:
5242438
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 84:14
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BLOOD COAGULATION FACTORS; AMINO ACID SEQUENCE; DNA SEQUENCING; GENES; BIOLOGICAL EVOLUTION; ATP; GLUCOPROTEINS; HYBRIDIZATION; LIVER; PHOSPHORUS 32; RECOMBINANT DNA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CARBOHYDRATES; COAGULANTS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DNA; DRUGS; GLANDS; HEMATOLOGIC AGENTS; ISOTOPES; LIGHT NUCLEI; MOLECULAR STRUCTURE; NUCLEI; NUCLEIC ACIDS; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; PROTEINS; RADIOISOTOPES; SACCHARIDES; STRUCTURAL CHEMICAL ANALYSIS; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Jenny, R J, Pittman, D D, Toole, J J, Kriz, R W, Aldape, R A, Hewick, R M, Kaufman, R J, and Mann, K G. Complete cDNA and derived amino acid sequence of human factor V. United States: N. p., 1987. Web. doi:10.1073/pnas.84.14.4846.
Jenny, R J, Pittman, D D, Toole, J J, Kriz, R W, Aldape, R A, Hewick, R M, Kaufman, R J, & Mann, K G. Complete cDNA and derived amino acid sequence of human factor V. United States. https://doi.org/10.1073/pnas.84.14.4846
Jenny, R J, Pittman, D D, Toole, J J, Kriz, R W, Aldape, R A, Hewick, R M, Kaufman, R J, and Mann, K G. 1987. "Complete cDNA and derived amino acid sequence of human factor V". United States. https://doi.org/10.1073/pnas.84.14.4846.
@article{osti_5242438,
title = {Complete cDNA and derived amino acid sequence of human factor V},
author = {Jenny, R J and Pittman, D D and Toole, J J and Kriz, R W and Aldape, R A and Hewick, R M and Kaufman, R J and Mann, K G},
abstractNote = {cDNA clones encoding human factor V have been isolated from an oligo(dT)-primed human fetal liver cDNA library prepared with vector Charon 21A. The cDNA sequence of factor V from three overlapping clones includes a 6672-base-pair (bp) coding region, a 90-bp 5' untranslated region, and a 163-bp 3' untranslated region within which is a poly(A)tail. The deduced amino acid sequence consists of 2224 amino acids inclusive of a 28-amino acid leader peptide. Direct comparison with human factor VIII reveals considerable homology between proteins in amino acid sequence and domain structure: a triplicated A domain and duplicated C domain show approx. 40% identity with the corresponding domains in factor VIII. As in factor VIII, the A domains of factor V share approx. 40% amino acid-sequence homology with the three highly conserved domains in ceruloplasmin. The B domain of factor V contains 35 tandem and approx. 9 additional semiconserved repeats of nine amino acids of the form Asp-Leu-Ser-Gln-Thr-Thr/Asn-Leu-Ser-Pro and 2 additional semiconserved repeats of 17 amino acids. Factor V contains 37 potential N-linked glycosylation sites, 25 of which are in the B domain, and a total of 19 cysteine residues.},
doi = {10.1073/pnas.84.14.4846},
url = {https://www.osti.gov/biblio/5242438}, journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 84:14,
place = {United States},
year = {Wed Jul 01 00:00:00 EDT 1987},
month = {Wed Jul 01 00:00:00 EDT 1987}
}