Complete cDNA and derived amino acid sequence of human factor V
cDNA clones encoding human factor V have been isolated from an oligo(dT)-primed human fetal liver cDNA library prepared with vector Charon 21A. The cDNA sequence of factor V from three overlapping clones includes a 6672-base-pair (bp) coding region, a 90-bp 5' untranslated region, and a 163-bp 3' untranslated region within which is a poly(A)tail. The deduced amino acid sequence consists of 2224 amino acids inclusive of a 28-amino acid leader peptide. Direct comparison with human factor VIII reveals considerable homology between proteins in amino acid sequence and domain structure: a triplicated A domain and duplicated C domain show approx. 40% identity with the corresponding domains in factor VIII. As in factor VIII, the A domains of factor V share approx. 40% amino acid-sequence homology with the three highly conserved domains in ceruloplasmin. The B domain of factor V contains 35 tandem and approx. 9 additional semiconserved repeats of nine amino acids of the form Asp-Leu-Ser-Gln-Thr-Thr/Asn-Leu-Ser-Pro and 2 additional semiconserved repeats of 17 amino acids. Factor V contains 37 potential N-linked glycosylation sites, 25 of which are in the B domain, and a total of 19 cysteine residues.
- Research Organization:
- Univ. of Vermont, Burlington (USA)
- OSTI ID:
- 5242438
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:14
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BLOOD COAGULATION FACTORS
AMINO ACID SEQUENCE
DNA SEQUENCING
GENES
BIOLOGICAL EVOLUTION
ATP
GLUCOPROTEINS
HYBRIDIZATION
LIVER
PHOSPHORUS 32
RECOMBINANT DNA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
COAGULANTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
DRUGS
GLANDS
HEMATOLOGIC AGENTS
ISOTOPES
LIGHT NUCLEI
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
550201* - Biochemistry- Tracer Techniques