The human gastrin/cholecystokinin type B receptor gene: Alternative splice donor site in exon 4 generates two variant mRNAs
- Univ. of Michigan Medical Center, Ann Arbor, MI (United States)
Gastrin and its carboxyl-terminal homolog cholecystokinin (CCK) exert a variety of biological actions in the brain and gastrointestinal tract that are mediated in part through one or more G protein-coupled receptors which exhibit similar affinity for both peptides. Genomic clones encoding a human gastrin/CCK[sub B] receptor were isolated by screening a human EMBL phage library with a partial-length DNA fragment which was based on the nucleotide sequence of the canine gastrin receptor. The gene contained a 1356-bp open reading frame consisting of five exons interrupted by 4 introns and was assigned to human chromosome 11p15.4. A region of exon 4, which encodes a portion of the putative third intracellular loop, appears to be alternatively spliced to yield two different mRNAs, one containing (452 aminio acids; long isoform) and the other lacking (447 amino acids; short isoform) the pentapeptide sequence Gly-Gly-Ala-Gly-Pro. The two receptor isoforms may contribute to functional differences in gastrin- and CCK-mediated signal transduction. 28 refs., 5 figs., 1 tab.
- OSTI ID:
- 5241383
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 90:19; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GENES
DNA SEQUENCING
GENETIC MAPPING
HUMAN CHROMOSOMES
RECEPTORS
DNA-CLONING
MESSENGER-RNA
RNA PROCESSING
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
HYBRIDIZATION
MAPPING
MEMBRANE PROTEINS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RNA
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics