Effect of manganese deficiency on wound healing glycosaminoglycans
- Texas Tech Univ., Lubbock (United States)
Manganese deficiency has been shown to depress proteglycan biosynthesis in the bone matrix in several species. Since the process of wound healing involves increased glycosaminoglycan (GAG) biosynthesis, the authors have made studies of the biosynthesis of GAGS in a wound healing model used in rats fed a diet deficient in manganese. Twelve female albino rats, 22-25 days old were divided into two groups of 6 each. One group was fed the manganese deficient diet; the second group was fed Purina Rodent diet. At maturation these females were mated with males on the Purina diet. Females were maintained on the same respective diets during gestation, delivery and lactation. From the offspring at weaning time, 12 males and 12 females were selected from each diet group. These animals were continued on the respective diets of their dams for 120 days. Each animals was then implanted with an acrylic wound healing cylinder. After 14 days each was injected with 20 microcuies of 1-{sup 14}C-glucosamine. After 24 hours, the cylinders were removed and tissue stripped from the inside of the cylinders. GAGS were separated by cellulose acetate electrophoresis and the radioactivity associated with each fraction determined. Weights of the tissue from the deficient group were significantly decreased. Chondroitin-4-sulfate and the radioactivity associated with this fraction were also decreased in the deficient group.
- OSTI ID:
- 5238196
- Report Number(s):
- CONF-9104107-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:5; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLYCOPROTEINS
BIOSYNTHESIS
MANGANESE
BIOLOGICAL EFFECTS
WOUNDS
HEALING
CARBON 14 COMPOUNDS
DIET
ELECTROPHORESIS
GLUCOSAMINE
METABOLISM
NUTRITIONAL DEFICIENCY
RATS
TRACER TECHNIQUES
AMINES
ANIMALS
BIOLOGICAL RECOVERY
CARBOHYDRATES
CARBON COMPOUNDS
ELEMENTS
HEXOSAMINES
HEXOSES
INJURIES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PROTEINS
RODENTS
SACCHARIDES
SYNTHESIS
TRANSITION ELEMENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology