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Title: Measurement of lung fluid volumes and albumin exclusion in sheep

Abstract

A radioactive tracer technique was used to determine interstitial diethylenetriaminepentaacetic acid (DTPA) and albumin distribution volume in sheep lungs. {sup 125}I- and/or {sup 131}I-labeled albumin were injected intravenously and allowed to equilibrate for 24 h. {sup 99m}Tc-labeled DTPA and {sup 51}Cr-labeled erythrocytes were injected and allowed to equilibrate (2 h and 15 min, respectively) before a lethal dose of thiamylal sodium. Two biopsies (1-3 g) were taken from each lung and the remaining tissue was homogenized for wet-to-dry lung weight and volume calculations. Estimates of distribution volumes from whole lung homogenized samples were statistically smaller than biopsy samples for extravascular water, interstitial {sup 99m}Tc-DTPA, and interstitial albumin. The mean fraction of the interstitium (Fe), which excludes albumin, was 0.68 +/- 0.04 for whole lung samples compared with 0.62 +/- 0.03 for biopsy samples. Hematocrit may explain the consistent difference. To make the Fe for biopsy samples match that for homogenized samples, a mean hematocrit, which was 82% of large vessel hematocrit, was required. Excluded volume fraction for exogenous sheep albumin was compared with that of exogenous human albumin in two sheep, and no difference was found at 24 h.

Authors:
; ; ; ;  [1]
  1. Vanderbilt Univ., Nashville, TN (USA)
Publication Date:
OSTI Identifier:
5228765
Resource Type:
Journal Article
Journal Name:
Journal of Applied Physiology (1985); (USA)
Additional Journal Information:
Journal Volume: 67:4; Journal ID: ISSN 8750-7587
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALBUMINS; TISSUE DISTRIBUTION; LUNGS; DYNAMIC FUNCTION STUDIES; BIOPSY; BLOOD COUNT; CHROMIUM 51; DTPA; ERYTHROCYTES; EXTRACELLULAR SPACE; IODINE 125; IODINE 131; ISOMERIC NUCLEI; SHEEP; TECHNETIUM 99; TRACER TECHNIQUES; AMINO ACIDS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CARBOXYLIC ACIDS; CHELATING AGENTS; CHROMIUM ISOTOPES; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; DISTRIBUTION; DOMESTIC ANIMALS; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; HOURS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOISOTOPES; RADIOPROTECTIVE SUBSTANCES; RESPIRATORY SYSTEM; RUMINANTS; SPACE; TECHNETIUM ISOTOPES; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Pou, N A, Roselli, R J, Parker, R E, Clanton, J A, and Harris, T R. Measurement of lung fluid volumes and albumin exclusion in sheep. United States: N. p., 1989. Web.
Pou, N A, Roselli, R J, Parker, R E, Clanton, J A, & Harris, T R. Measurement of lung fluid volumes and albumin exclusion in sheep. United States.
Pou, N A, Roselli, R J, Parker, R E, Clanton, J A, and Harris, T R. 1989. "Measurement of lung fluid volumes and albumin exclusion in sheep". United States.
@article{osti_5228765,
title = {Measurement of lung fluid volumes and albumin exclusion in sheep},
author = {Pou, N A and Roselli, R J and Parker, R E and Clanton, J A and Harris, T R},
abstractNote = {A radioactive tracer technique was used to determine interstitial diethylenetriaminepentaacetic acid (DTPA) and albumin distribution volume in sheep lungs. {sup 125}I- and/or {sup 131}I-labeled albumin were injected intravenously and allowed to equilibrate for 24 h. {sup 99m}Tc-labeled DTPA and {sup 51}Cr-labeled erythrocytes were injected and allowed to equilibrate (2 h and 15 min, respectively) before a lethal dose of thiamylal sodium. Two biopsies (1-3 g) were taken from each lung and the remaining tissue was homogenized for wet-to-dry lung weight and volume calculations. Estimates of distribution volumes from whole lung homogenized samples were statistically smaller than biopsy samples for extravascular water, interstitial {sup 99m}Tc-DTPA, and interstitial albumin. The mean fraction of the interstitium (Fe), which excludes albumin, was 0.68 +/- 0.04 for whole lung samples compared with 0.62 +/- 0.03 for biopsy samples. Hematocrit may explain the consistent difference. To make the Fe for biopsy samples match that for homogenized samples, a mean hematocrit, which was 82% of large vessel hematocrit, was required. Excluded volume fraction for exogenous sheep albumin was compared with that of exogenous human albumin in two sheep, and no difference was found at 24 h.},
doi = {},
url = {https://www.osti.gov/biblio/5228765}, journal = {Journal of Applied Physiology (1985); (USA)},
issn = {8750-7587},
number = ,
volume = 67:4,
place = {United States},
year = {Sun Oct 01 00:00:00 EDT 1989},
month = {Sun Oct 01 00:00:00 EDT 1989}
}