skip to main content

Title: Altered catecholamine receptor affinity in rabbit aortic intimal hyperplasia

Intimal thickening is a universal response to endothelial denudation and is also thought to be a precursor of atherosclerosis. The authors have demonstrated selective supersensitivity in arterial intimal hyperplasia to norepinephrine and they now report a possible mechanism for this. Binding studies in rabbit aorta with the selective alpha 1-adrenergic radioligand 125I-HEAT demonstrated that there was no change in receptor density (20 {plus minus} 4 fmole/10(6) cells) in intact vascular smooth muscle cells at either 5 or 14 days after denudation. However, competition studies showed a 2.6-fold increase in alpha 1-adrenergic receptor affinity for norepinephrine in intimal hyperplastic tissue (P less than 0.05). This increased affinity for norepinephrine was associated with a greater increase in 32P-labeled phosphatidylinositol (148% intimal thickening versus 76% control) and phosphatidic acid (151% intimal thickening versus 56% control) following norepinephrine stimulation of free floating rings of intimal hyperplastic aorta. These data suggest that the catecholamine supersensitivity in rabbit aortic intimal hyperplasia is receptor mediated and may be linked to the phosphatidylinositol cycle.
Authors:
; ;  [1]
  1. (Duke University Medical Center, Durham, NC (USA))
Publication Date:
OSTI Identifier:
5227804
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Surgical Research; (United States); Journal Volume: 51:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CATECHOLAMINES; RECEPTORS; VASCULAR DISEASES; PATHOGENESIS; AFFINITY; AORTA; BIOCHEMICAL REACTION KINETICS; IODINE 125; LIGANDS; MUSCLES; NORADRENALINE; PHOSPHOLIPIDS; PHOSPHORUS 32; RABBITS; TRACER TECHNIQUES; ADRENAL HORMONES; AMINES; ANIMALS; AROMATICS; ARTERIES; AUTONOMIC NERVOUS SYSTEM AGENTS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BLOOD VESSELS; BODY; CARDIOTONICS; CARDIOVASCULAR AGENTS; CARDIOVASCULAR SYSTEM; DAYS LIVING RADIOISOTOPES; DISEASES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; ESTERS; HORMONES; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LIGHT NUCLEI; LIPIDS; MAMMALS; MEMBRANE PROTEINS; NEUROREGULATORS; NUCLEI; ODD-EVEN NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; ORGANS; PHENOLS; PHOSPHORUS ISOTOPES; POLYPHENOLS; PROTEINS; RADIOISOTOPES; REACTION KINETICS; SYMPATHOMIMETICS; VERTEBRATES 550901* -- Pathology-- Tracer Techniques