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Title: Characterization of the interaction between local cerebral metabolic rate for glucose and acid-base index in ischemic rat brain employing a double-isotope methodology

The association between increases in cerebral glucose metabolism and the development of acidosis is largely inferential, based on reports linking hyperglycemia with poor neurological outcome, lactate accumulation, and the severity of acidosis. We measured local cerebral metabolic rate for glucose (lCMRglc) and an index of brain pH-the acid-base index (ABI)-concurrently and characterized their interaction in a model of focal cerebral ischemia in rats in a double-label autoradiographic study, using ({sup 14}C)2-deoxyglucose and ({sup 14}C)dimethyloxazolidinedione. Computer-assisted digitization and analysis permitted the simultaneous quantification of the two variables on a pixel-by-pixel basis in the same brain slices.
Authors:
Publication Date:
OSTI Identifier:
5227316
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Publisher:
Houston, TX (US); Univ. of Texas
Research Org:
Texas Univ., Houston, TX (USA). Health Science Center
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GLUCOSE; METABOLISM; ISCHEMIA; PATHOGENESIS; AUTORADIOGRAPHY; CARBON 14 COMPOUNDS; CEREBRAL CORTEX; CHEMICAL COMPOSITION; COMPUTER CALCULATIONS; DOUBLE LABELLING; HYPERGLYCEMIA; PH VALUE; RATS; ALDEHYDES; ANIMALS; BODY; BRAIN; CARBOHYDRATES; CARDIOVASCULAR DISEASES; CENTRAL NERVOUS SYSTEM; CEREBRUM; DISEASES; HEXOSES; LABELLED COMPOUNDS; LABELLING; MAMMALS; MONOSACCHARIDES; NERVOUS SYSTEM; ORGANIC COMPOUNDS; ORGANS; RODENTS; SACCHARIDES; VASCULAR DISEASES; VERTEBRATES 550501* -- Metabolism-- Tracer Techniques; 550901 -- Pathology-- Tracer Techniques